Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Rabbit prostaglandin omega-hydroxylase (CYP4A4) - gene structure and expression

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Palmer, C. N. A.
  • Griffin, K. J.
  • Johnson, Eric

publication date

  • 1993

journal

  • Archives of Biochemistry and Biophysics  Journal

abstract

  • Genomic DNA containing the entire gene encoding P450 4A4, a cytochrome P450 that is elevated during pregnancy, was isolated on two overlapping recombinant phage. The isolated gene, CYP4A4, encodes a protein that differs at one amino acid position from the predicted sequence of a previously isolated cDNA. The intron/exon structure is highly conserved in relation to the clofibrate inducible genes CYP4A1, CYP4A2, and CYP4A6. However, the 5' flanking sequences of these genes exhibit little identity. Two transcriptional start sites of the CYP4A4 gene have been determined by RNase protection analysis and are located 37 and 40 nucleotides upstream of the initiation codon. Like other CYP4A genes the CYP4A4 promoter does not contain a TATA consensus sequence. CYP4A4 mRNAs are not detected in the lungs, liver, and kidneys of untreated rabbits by RNase protection assays. However, CYP4A4 mRNA is present to varying degrees in all three of these tissues during pregnancy with the greatest abundance observed in the lung and the lowest in the kidney. Treatment of male rabbits with dexamethasone also increases the levels of CYP4A4 mRNA in the lung and liver, but the levels are eightfold less than those seen for pregnant rabbits. Consensus recognition sequences for either the glucocorticoid or progesterone receptors were not found in 1086 bp of sequence upstream of the start of transcription.

subject areas

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Clofibrate
  • Cloning, Molecular
  • Cytochrome P-450 Enzyme System
  • Dexamethasone
  • Enzyme Induction
  • Exons
  • Female
  • Gene Expression Regulation, Enzymologic
  • Genomic Library
  • Introns
  • Kidney
  • Liver
  • Lung
  • Male
  • Mixed Function Oxygenases
  • Molecular Sequence Data
  • Oligonucleotides, Antisense
  • Pregnancy
  • RNA, Messenger
  • Rabbits
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • TATA Box
  • Transcription, Genetic
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0003-9861

Digital Object Identifier (DOI)

  • 10.1006/abbi.1993.1093

PubMed ID

  • 8434947
scroll to property group menus

Additional Document Info

start page

  • 670

end page

  • 676

volume

  • 300

issue

  • 2

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support