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Transduction of human CD34+ cells that mediate long-term engraftment of NOD/SCID mice by HIV vectors

Academic Article
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Overview

authors

  • Miyoshi, H.
  • Smith, K. A.
  • Mosier, Donald
  • Verma, I. M.
  • Torbett, Bruce

publication date

  • January 1999

journal

  • Science  Journal

abstract

  • Efficient gene transfer into human hematopoietic stem cells (HSCs) is an important goal in the study of the hematopoietic system as well as for gene therapy of hematopoietic disorders. A lentiviral vector based on the human immunodeficiency virus (HIV) was able to transduce human CD34+ cells capable of stable, long-term reconstitution of nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. High-efficiency transduction occurred in the absence of cytokine stimulation and resulted in transgene expression in multiple lineages of human hematopoietic cells for up to 22 weeks after transplantation.

subject areas

  • Animals
  • Antigens, CD34
  • Bone Marrow Cells
  • Cell Division
  • Cell Survival
  • Colony-Forming Units Assay
  • Gene Expression
  • Gene Transfer Techniques
  • Genetic Vectors
  • Green Fluorescent Proteins
  • HIV
  • Hematopoiesis
  • Hematopoietic Stem Cell Transplantation
  • Hematopoietic Stem Cells
  • Humans
  • Leukemia Virus, Murine
  • Luminescent Proteins
  • Mice
  • Mice, Inbred NOD
  • Mice, SCID
  • Promoter Regions, Genetic
  • Transfection
  • Transgenes
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Identity

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.283.5402.682

PubMed ID

  • 9924027
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Additional Document Info

start page

  • 682

end page

  • 686

volume

  • 283

issue

  • 5402

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