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Thp-1 cells form foam cells in response to coculture with lipoproteins but not platelets

Academic Article
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Overview

authors

  • Banka, C. L.
  • Black, A. S.
  • Dyer, C. A.
  • Curtiss, Linda

publication date

  • 1991

journal

  • Journal of Lipid Research  Journal

abstract

  • The human monocytic leukemia cell line, THP-1, shares many properties with human monocyte-derived macrophages and might be a useful model for studying foam cell formation in vitro. Therefore, we examined the ability of THP-1 cells to accumulate cholesteryl esters, the hallmark feature of foam cells, in response to culture with native low density lipoprotein (LDL), modified LDL, and platelets. THP-1 cells stored more cholesteryl esters than macrophages in response to 200 micrograms/ml of LDL. Down-regulation of LDL receptors occurred in macrophages at lower LDL concentrations than in THP-1 cells. Phorbol ester-treated THP-1 cells stored more cholesteryl esters than human macrophages in response to 25-200 micrograms/ml of acetylated LDL. Because we have previously demonstrated that activated platelets enhanced macrophage cholesteryl ester storage, we examined the ability of THP-1 cells to store cholesteryl esters in response to coculture with platelets. Compared with macrophages, dividing THP-1 cells and phorbol ester-treated THP-1 cells accumulated only 50% and 33% as much cholesteryl esters, respectively. Furthermore, although platelets induced a 90% reduction in cholesterol synthesis in macrophages by day 5, cholesterol synthesis in THP-1 cells and phorbol ester-treated THP-1 cells was inhibited less than 50% by platelets. Nevertheless, both THP-1 cells and macrophages responded to platelets by increasing their secretion of apolipoprotein E. Therefore, we conclude that dividing THP-1 cells and phorbol ester-treated THP-1 cells are capable of forming foam cells in response to physiologic doses of both LDL and acetylated LDL, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

subject areas

  • Apolipoproteins E
  • Blood Platelets
  • Cholesterol
  • Cholesterol Esters
  • Esterification
  • Foam Cells
  • Humans
  • Kinetics
  • Lipoproteins, LDL
  • Receptors, LDL
  • Tumor Cells, Cultured
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Research

keywords

  • ACETYLATED LDL
  • CHOLESTEROL
  • LDL
  • MACROPHAGE
  • MONOCYTE
  • PHORBOL ESTER
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Identity

International Standard Serial Number (ISSN)

  • 0022-2275

PubMed ID

  • 2010692
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Additional Document Info

start page

  • 35

end page

  • 43

volume

  • 32

issue

  • 1

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