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Combined pseudo-merohedral twinning, non-crystallographic symmetry and pseudo-translation in a monoclinic crystal form of the gamma delta t-cell ligand t10

Academic Article
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Overview

authors

  • Rudolph, M. G.
  • Wingren, C.
  • Crowley, M. P.
  • Chien, Y. H.
  • Wilson, Ian

publication date

  • April 2004

journal

  • Acta Crystallographica Section D-Biological Crystallography  Journal

abstract

  • T10 is a non-classical class Ib-like major histocompatibility complex (MHC) cell-surface antigen which binds directly to certain gammadelta T-cell receptors in the absence of any exogenous and endogenous ligands, such as peculiar lipids or glycolipids. The crystal structure at 2.5 A resolution of murine T10 was determined by molecular replacement using data from an almost perfectly twinned monoclinic crystal. The space group is P2(1), with unit-cell parameters a = 78.2, b = 70.0, c = 139.2 A, beta = 106.8 degrees. Self-rotation function analysis and various intensity statistics revealed the presence of pseudo-merohedral twinning, but these tests underestimated the true twin fraction of alpha approximately 0.46. Native Patterson analyses pointed to the presence of pseudo-translation among the four molecules present in the asymmetric unit. Data analysis, structure determination and model refinement are discussed.

subject areas

  • Animals
  • Antigens, Surface
  • Crystallization
  • Crystallography, X-Ray
  • H-2 Antigens
  • Mice
  • Models, Molecular
  • Molecular Structure
  • Protein Conformation
  • Receptors, Antigen, T-Cell, gamma-delta
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Identity

International Standard Serial Number (ISSN)

  • 0907-4449

Digital Object Identifier (DOI)

  • 10.1107/s0907444904002239

PubMed ID

  • 15039553
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Additional Document Info

start page

  • 656

end page

  • 664

volume

  • 60

issue

  • Pt 4

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