NMR assignment of the domain 513-651 from the SARS-CoV nonstructural protein nsp3

Academic Article
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publication date

  • December 2007

abstract

  • Sequence-specific NMR assignments of an internal domain of the protein nsp3, nsp3(513-651), which is a part of the SARS coronavirus (SARS-CoV) replicase polyprotein, have been determined, using triple-resonance NMR experiments with the uniformly [(13)C,(15)N]-labeled protein. The complete assignments (>99%) provide the basis for the ongoing three-dimensional structure determination.