recent publications

• academic article

  • Kovarik, Z., Hrvat, N. M., Katalinic, M., Sit, R. K., Paradyse, A., Zunec, S., Musilek, K., Fokin, V. V., Taylor, P., Radic, Z. Catalytic soman scavenging by the Y337A/F338A acetylcholinesterase mutant assisted with novel site-directed aldoximes Chemical Research in Toxicology  2015 28:1036-1044  DOI:10.1021/acs.chemrestox.5b00060  PMID:25835984  PMCID:PMC4791098
  • Arunrungichian, K., Boonyarat, C., Fokin, V. V., Taylor, P., Vajragupta, O. Cognitive improvements in a mouse model with substituted 1,2,3-triazole agonists for nicotinic acetylcholine receptors ACS Chemical Neuroscience  2015 6:1331-1340  DOI:10.1021/acschemneuro.5b00059  PMID:25978789
  • Arunrungvichian, K., Fokin, V. V., Vajragupta, O., Taylor, P. Selectivity optimization of substituted 1,2,3-triazoles as α7 nicotinic acetylcholine receptor agonists ACS Chemical Neuroscience  2015 6:1317-1330  DOI:10.1021/acschemneuro.5b00058  PMID:25932897
  • Sit, R. K., Fokin, V. V., Amitai, G., Sharpless, K. B., Taylor, P., Radic, Z. Imidazole aldoximes effective in assisting butyrylcholinesterase catalysis of organophosphate detoxification Journal of Medicinal Chemistry  2014 57:1378-1389  DOI:10.1021/jm401650z  PMID:24571195  PMCID:PMC4167068
  • Kaczanowska, K., Harel, M., Radic, Z., Changeux, J. P., Finn, M. G., Taylor, P. Structural basis for cooperative interactions of substituted 2-aminopyrimidines with the acetylcholine binding protein Proceedings of the National Academy of Sciences of the United States of America  2014 111:10749-10754  DOI:10.1073/pnas.1410992111  PMID:25006260  PMCID:PMC4115551
  • Morris, G. M., Green, L. G., Radic, Z., Taylor, P., Sharpless, K. B., Olson, A. J., Grynszpan, F. Automated docking with protein flexibility in the design of femtomolar "click chemistry" inhibitors of acetylcholinesterase Journal of Chemical Information and Modeling  2013 53:898-906  DOI:10.1021/ci300545a  PMID:23451944  PMCID:PMC3698963
  • Radic, Z., Dale, T., Kovarik, Z., Berend, S., Garcia, E., Zhang, L. M., Amitai, G., Green, C., Radic, B., Duggan, B. M., Ajami, D., Rebek, J., et al. Catalytic detoxification of nerve agent and pesticide organophosphates by butyrylcholinesterase assisted with non-pyridinium oximes Biochemical Journal  2013 450:231-242  DOI:10.1042/bj20121612  PMID:23216060
  • Kovarik, Z., Macek, N., Sit, R. K., Radic, Z., Fokin, V. V., Sharpless, K. B., Taylor, P. Centrally acting oximes in reactivation of tabun-phosphoramidated AChE Chemico-Biological Interactions  2013 203:77-80  DOI:10.1016/j.cbi.2012.08.019  PMID:22960624  PMCID:PMC3530643
  • Radic, Z., Sit, R. K., Garcia, E., Zhang, L., Berend, S., Kovarik, Z., Amitai, G., Fokin, V. V., Sharpless, K. B., Taylor, P. Mechanism of interaction of novel uncharged, centrally active reactivators with OP-hAChE conjugates Chemico-Biological Interactions  2013 203:67-71  DOI:10.1016/j.cbi.2012.08.014  PMID:22975155  PMCID:PMC3561488
  • Grimster, N. P., Stump, B., Fotsing, J. R., Weide, T., Talley, T. T., Yamauchi, J. G., Nemecz, A., Kim, C., Ho, K. Y., Sharpless, K. B., Taylor, P., Fokin, V. V. Generation of candidate ligands for nicotinic acetylcholine receptors via in situ click chemistry with a soluble acetylcholine binding protein template Journal of the American Chemical Society  2012 134:6732-6740  DOI:10.1021/ja3001858  PMID:22394239  PMCID:PMC3618991
  • Radic, Z., Sit, R. K., Kovarik, Z., Berend, S., Garcia, E., Zhang, L. M., Amitai, G., Green, C., Radic, B., Fokin, V. V., Sharpless, K. B., Taylor, P. Refinement of structural leads for centrally acting oxime reactivators of phosphylated cholinesterases Journal of Biological Chemistry  2012 287:11798-11809  DOI:10.1074/jbc.M111.333732  PMID:22343626  PMCID:PMC3320928
  • Yamauchi, J. G., Gomez, K., Grimster, N., Dufouil, M., Nemecz, A., Fotsing, J. R., Ho, K. Y., Talley, T. T., Sharpless, K. B., Fokin, V. V., Taylor, P. Synthesis of selective agonists for the alpha 7 nicotinic acetylcholine receptor with in situ click-chemistry on acetylcholine-binding protein templates Molecular Pharmacology  2012 82:687-699  DOI:10.1124/mol.112.080291  PMID:22784805  PMCID:PMC3463225
  • Sit, R. K., Radic, Z., Gerardi, V., Zhang, L. M., Garcia, E., Katalinic, M., Amitai, G., Kovarik, Z., Fokin, V. V., Sharpless, K. B., Taylor, P. New structural scaffolds for centrally acting oxime reactivators of phosphylated cholinesterases Journal of Biological Chemistry  2011 286:19422-19430  DOI:10.1074/jbc.M111.230656  PMID:21464125  PMCID:PMC3103321
  • Cochran, R., Kalisiak, J., Kucukkilinc, T., Radic, Z., Garcia, E., Zhang, L. M., Ho, K. Y., Amitai, G., Kovarik, Z., Fokin, V. V., Sharpless, K. B., Taylor, P. Oxime-assisted acetylcholinesterase catalytic scavengers of organophosphates that resist aging Journal of Biological Chemistry  2011 286:29718-29724  DOI:10.1074/jbc.M111.264739  PMID:21730071  PMCID:PMC3191013
  • Miller, M. T., Mileni, M., Comoletti, D., Stevens, R. C., Harel, M., Taylor, P. The crystal structure of the alpha-neurexin-1 extracellular region reveals a hinge point for mediating synaptic adhesion and function Structure  2011 19:767-778  DOI:10.1016/j.str.2011.03.011  PMID:21620717  PMCID:PMC3279696
  • Radic, Z., Kalisiak, J., Fokin, V. V., Sharpless, K. B., Taylor, P. Interaction kinetics of oximes with native, phosphylated and aged human acetylcholinesterase Chemico-Biological Interactions  2010 187:163-166  DOI:10.1016/j.cbi.2010.04.014  PMID:20412789  PMCID:PMC2912944
  • Sine, S. M., Wang, H. L., Hansen, S., Taylor, P. On the origin of ion selectivity in the Cys-loop receptor family Journal of Molecular Neuroscience  2010 40:70-76  DOI:10.1007/s12031-009-9260-1  PMID:19728176  PMCID:PMC3047408
  • de Wit, J., Sylwestrak, E., O'Sullivan, M. L., Otto, S., Tiglio, K., Savas, J. N., Yates, J. R., Comoletti, D., Taylor, P., Ghosh, A. LRRTM2 interacts with Neurexin1 and regulates excitatory synapse formation Neuron  2009 64:799-806  DOI:10.1016/j.neuron.2009.12.019  PMID:20064388  PMCID:PMC2829666
  • Rana, B. K., Wessel, J., Mahboubi, V., Rao, F. W., Haeller, J., Gayen, J. R., Eskin, E., Valle, A. M., Das, M., Mahata, S. K., Taupenot, L., Stridsberg, M., et al. Natural variation within the neuronal nicotinic acetylcholine receptor cluster on human chromosome 15q24: Influence on heritable autonomic traits in twin pairs Journal of Pharmacology and Experimental Therapeutics  2009 331:419-428  DOI:10.1124/jpet.109.157271  PMID:19671882  PMCID:PMC2775266
  • Hansen, S. B., Wang, H. L., Taylor, P., Sine, S. M. An ion selectivity filter in the extracellular domain of Cys-loop receptors reveals determinants for ion conductance Journal of Biological Chemistry  2008 283:36066-36070  DOI:10.1074/jbc.C800194200  PMID:18940802  PMCID:PMC2662287
  • Radic, Z., Manetsch, R., Fournier, D., Sharpless, K. B., Taylor, P. Probing gorge dimensions of cholinesterases by freeze-frame click chemistry Chemico-Biological Interactions  2008 175:161-165  DOI:10.1016/j.cbi.2008.04.048  PMID:18555981  PMCID:PMC2585062
  • Hansen, S. B., Taylor, P. Galanthamine and non-competitive inhibitor binding to ACh-binding protein: evidence for a binding site on non-alpha-subunit interfaces of heteromeric neuronal nicotinic receptors Journal of Molecular Biology  2007 369:895-901  DOI:10.1016/j.jmb.2007.03.067  PMID:17481657  PMCID:PMC2031909
  • Taylor, P., Talley, T. T., Radic, Z., Hansen, S. B., Hibbs, R. E., Shi, J. Structure-guided drug design: Conferring selectivity among neuronal nicotinic receptor and acetylcholine-binding protein subtypes Biochemical Pharmacology  2007 74:1164-1171  DOI:10.1016/j.bcp.2007.07.038  PMID:17826748  PMCID:PMC3341175
  • Gao, F., Mer, G., Tonelli, M., Hansen, S. B., Burghardt, T. P., Taylor, P., Sine, S. M. Solution NMR of acetylcholine binding protein reveals agonist-mediated conformational change of the C-loop Molecular Pharmacology  2006 70:1230-1235  DOI:10.1124/mol.106.027185  PMID:16847142
  • Hansen, S. B., Suizenbacher, G., Huxford, T., Marchot, P., Bourne, Y., Taylor, P. Structural characterization of agonist and antagonist-bound acetylcholine-binding protein from Aplysia californica Journal of Molecular Neuroscience  2006 30:101-102  DOI:10.1385/JMN:30:1:101  PMID:17192647
  • Bourne, Y., Hansen, S. B., Sulzenbacher, G., Talley, T. T., Huxford, T., Taylor, P., Marchot, P. Structural comparison of three crystalline complexes of a peptidic toxin with a synaptic acetylcholine recognition protein Journal of Molecular Neuroscience  2006 30:103-104  DOI:10.1385/JMN:30:1:103  PMID:17192648  PMCID:PMC4755294
  • Gao, F., Bren, N., Burghardt, T. P., Hansen, S., Henchman, R. H., Taylor, P., McCammon, J. A., Sine, S. M. Agonist-mediated conformational changes in acetylcholine-binding protein revealed by simulation and intrinsic tryptophan fluorescence Journal of Biological Chemistry  2005 280:8443-8451  DOI:10.1074/jbc.M412389200  PMID:15591050
  • Bourne, Y., Talley, T. T., Hansen, S. B., Taylor, P., Marchot, P. Crystal structure of a Cbtx-AChBP complex reveals essential interactions between snake alpha-neurotoxins and nicotinic receptors EMBO Journal  2005 24:1512-1522  DOI:10.1038/sj.emboj.7600620  PMID:15791209  PMCID:PMC1142565
  • Krasinski, A., Radic, Z., Manetsch, R., Raushel, J., Taylor, P., Sharpless, K. B., Kolb, H. C. In situ selection of lead compounds by click chemistry: Target-guided optimization of acetylcholinesterase inhibitors Journal of the American Chemical Society  2005 127:6686-6692  DOI:10.1021/ja0430311  PMID:15869290
  • Radic, Z., Manetsch, R., Krasinski, A., Raushel, J., Yamauchi, J., Garcia, C., Kolb, H., Sharpless, K. B., Taylor, P. Molecular basis of interactions of cholinesterases with tight binding inhibitors Chemico-Biological Interactions  2005 157:133-141  DOI:10.1016/j.cbi.2005.10.020  PMID:16289416
  • Hibbs, R. E., Johnson, D. A., Shi, J. X., Hansen, S. B., Taylor, P. Structural dynamics of the alpha-neurotoxin-acetylcholine-binding protein complex: Hydrodynamic and fluorescence anisotropy decay analyses Biochemistry  2005 44:16602-16611  DOI:10.1021/bi051735p  PMID:16342951
  • Bourne, Y., Radic, Z., Kolb, H. C., Sharpless, K. B., Taylor, P., Marchot, P. Structural insights into conformational flexibility at the peripheral site and within the active center gorge of ache Chemico-Biological Interactions  2005 157:159-165  DOI:10.1016/j.cbi.2005.10.018  PMID:16259971
  • Hansen, S. B., Sulzenbacher, G., Huxford, T., Marchot, P., Taylor, P., Bourne, Y. Structures of Aplysia AChBP complexes with nicotinic agonists and antagonists reveal distinctive binding interfaces and conformations EMBO Journal  2005 24:3635-3646  DOI:10.1038/sj.emboj.7600828  PMID:16193063  PMCID:PMC1276711
  • Taylor, P., Hansen, S. B., Talley, T. T., Hibbs, R. E., Radic, Z. Contemporary paradigms for cholinergic ligand design guided by biological structure Bioorganic & Medicinal Chemistry Letters  2004 14:1875-1877  DOI:10.1016/j.bmcl.2003.10.072  PMID:15050619  PMCID:PMC3191913
  • Bouzat, C., Gumilar, F., Spitzmaul, G., Wang, H. L., Rayes, D., Hansen, S. B., Taylor, P., Sine, S. M. Coupling of agonist binding to channel gating in an ACh-binding protein linked to an ion channel Nature  2004 430:896-900  DOI:10.1038/nature02753  PMID:15318223
  • Bourne, Y., Kolb, H. C., Radic, Z., Sharpless, K. B., Taylor, P., Marchot, P. Freeze-frame inhibitor captures acetylcholinesterase in a unique conformation Proceedings of the National Academy of Sciences of the United States of America  2004 101:1449-1454  DOI:10.1073/pnas.0308206100  PMID:14757816  PMCID:PMC341740
  • Manetsch, R., Krasinski, A., Radic, Z., Raushel, J., Taylor, P., Sharpless, K. B., Kolb, H. C. In situ click chemistry: Enzyme inhibitors made to their own specifications Journal of the American Chemical Society  2004 126:12809-12818  DOI:10.1021/ja046382g  PMID:15469276
  • Hansen, S. B., Talley, T. T., Radic, Z., Taylor, P. Structural and ligand recognition characteristics of an acetylcholine-binding protein from Aplysia californica Journal of Biological Chemistry  2004 279:24197-24202  DOI:10.1074/jbc.M402452200  PMID:15069068  PMCID:PMC4762456
  • Gao, F., Bren, N., Little, A., Wang, H. L., Hansen, S. B., Talley, T. T., Taylor, P., Sine, S. M. Curariform antagonists bind in different orientations to acetylcholine-binding protein Journal of Biological Chemistry  2003 278:23020-23026  DOI:10.1074/jbc.M301151200  PMID:12682067  PMCID:PMC3191914
  • Lewis, W. G., Green, L. G., Grynszpan, F., Radic, Z., Carlier, P. R., Taylor, P., Finn, M. G., Sharpless, K. B. Click chemistry in situ: Acetylcholinesterase as a reaction vessel for the selective assembly of a femtomolar inhibitor from an array of building blocks Angewandte Chemie-International Edition  2002 41:1053-1057  DOI:10.1002/1521-3773(20020315)41:6<1053::aid-anie1053>3.0.co;2-4  PMID:12491310
  • Hansen, S. B., Radic, Z., Talley, T. T., Molles, B. E., Deerinck, T., Tsigelny, I., Taylor, P. Tryptophan fluorescence reveals conformational changes in the acetylcholine binding protein Journal of Biological Chemistry  2002 277:41299-41302  DOI:10.1074/jbc.C200462200  PMID:12235129  PMCID:PMC3191908