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Isolation and angiogenesis by endothelial progenitors in the fetal liver

Academic Article
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Overview

authors

  • Cherqui, Stephanie
  • Kurian, S. M.
  • Schussler, O.
  • Hewel, J. A.
  • Yates III, John
  • Salomon, Daniel

publication date

  • January 2006

journal

  • Stem Cells  Journal

abstract

  • Endothelial progenitor cells (EPCs) have significant therapeutic potential. However, the low quantity of such cells available from bone marrow and their limited capacity to proliferate in culture make their use difficult. Here, we present the first definitive demonstration of the presence of true EPCs in murine fetal liver capable of forming blood vessels in vivo connected to the host's vasculature after transplantation. This population is particularly interesting because it can be obtained at high yield and has a high angiogenic capacity as compared with bone marrow-derived EPCs. The EPC capacity is contained within the CD31+Sca1+ cell subset. We demonstrate that these cells are dependent for survival and proliferation on a feeder cell monolayer derived from the fetal liver. In addition, we describe a novel and easy method for the isolation and ex vivo proliferation of these EPCs. Finally, we used gene expression profiling and tandem mass spectrometry proteomics to examine the fetal liver endothelial progenitors and the feeder cells to identify possible proangiogenic growth factor and endothelial differentiation-associated genes.

subject areas

  • Animals
  • Antigens, CD31
  • Antigens, Ly
  • Cell Differentiation
  • Cell Separation
  • Endothelial Cells
  • Gene Expression Profiling
  • In Vitro Techniques
  • Liver
  • Membrane Proteins
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, SCID
  • Mice, Transgenic
  • Neovascularization, Physiologic
  • Phenotype
  • Stem Cell Transplantation
  • Stem Cells
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Research

keywords

  • CD31(+)Sca1(+) cells
  • angiogenesis
  • endothelial progenitor cells
  • murine fetal liver
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Identity

International Standard Serial Number (ISSN)

  • 1066-5099

Digital Object Identifier (DOI)

  • 10.1634/stemcells.2005-0070

PubMed ID

  • 16099996
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Additional Document Info

start page

  • 44

end page

  • 54

volume

  • 24

issue

  • 1

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