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The quorum quenching antibody RS2-1G9 protects macrophages from the cytotoxic effects of the pseudomonas aeruginosa quorum sensing signalling molecule N-3-oxo-dodecanoyl-homoserine lactone

Academic Article
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Overview

authors

  • Kaufmann, Gunnar
  • Park, J.
  • Mee, J. M.
  • Ulevitch, Richard
  • Janda, Kim

publication date

  • May 2008

journal

  • Molecular Immunology  Journal

abstract

  • The Gram-negative bacterium Pseudomonas aeruginosa, an opportunistic human pathogen, uses acyl-homoserine lactone-based quorum sensing systems to control its pathogenicity. One of its quorum sensing factors, N-3-oxo-dodecanoyl-homoserine lactone, has been shown not only to mediate bacterial quorum sensing but also to exert cytotoxic effects on mammalian cells. The monoclonal antibody RS2-1G9 generated against a 3-oxo-dodecanoyl-homoserine lactone analogue hapten was able to protect murine bone marrow-derived macrophages from the cytotoxic effects and also prevented the activation of the mitogen-activated protein kinase p38. These data demonstrate that an immunopharmacotherapeutic approach to combat P. aeruginosa infections might be a viable therapeutic option as the monoclonal antibody RS2-1G9 can readily sequester bacterial N-3-oxo-dodecanoyl-homoserine lactone molecules, thus interfering with their biological effects in prokaryotic and eukaryotic systems.

subject areas

  • 4-Butyrolactone
  • Animals
  • Antibodies, Monoclonal
  • Cell Line
  • Cells, Cultured
  • Enzyme Activation
  • Haptens
  • Homoserine
  • Macrophages
  • Mice
  • Poly(ADP-ribose) Polymerases
  • Pseudomonas aeruginosa
  • Quorum Sensing
  • p38 Mitogen-Activated Protein Kinases
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Research

keywords

  • Pseudomonas aeruginosa
  • acyl-homoserine lactone
  • immunopharmacotherapy
  • monoclonal antibody
  • quorum sensing
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Identity

PubMed Central ID

  • PMC2359578

International Standard Serial Number (ISSN)

  • 0161-5890

Digital Object Identifier (DOI)

  • 10.1016/j.molimm.2008.01.010

PubMed ID

  • 18304641
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Additional Document Info

start page

  • 2710

end page

  • 2714

volume

  • 45

issue

  • 9

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