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Antigen-specific b cell memory: Expression and replenishment of a novel b220(-) memory b cell compartment

Academic Article
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Overview

authors

  • McHeyzer-Williams, L. J.
  • Cool, M.
  • McHeyzer-Williams, Michael G.

publication date

  • April 2000

journal

  • Journal of Experimental Medicine  Journal

abstract

  • The mechanisms that regulate B cell memory and the rapid recall response to antigen remain poorly defined. This study focuses on the rapid expression of B cell memory upon antigen recall in vivo, and the replenishment of quiescent B cell memory that follows. Based on expression of CD138 and B220, we reveal a unique and major subtype of antigen-specific memory B cells (B220(-)CD138(-)) that are distinct from antibody-secreting B cells (B220(+/)-CD138(+)) and B220(+)CD138(-) memory B cells. These nonsecreting somatically mutated B220(-) memory responders rapidly dominate the splenic response and comprise >95% of antigen-specific memory B cells that migrate to the bone marrow. By day 42 after recall, the predominant quiescent memory B cell population in the spleen (75-85%) and the bone marrow (>95%) expresses the B220(-) phenotype. Upon adoptive transfer, B220(-) memory B cells proliferate to a lesser degree but produce greater amounts of antibody than their B220(+) counterparts. The pattern of cellular differentiation after transfer indicates that B220(-) memory B cells act as stable self-replenishing intermediates that arise from B220(+) memory B cells and produce antibody-secreting cells on rechallenge with antigen. Cell surface phenotype and Ig isotype expression divide the B220(-) compartment into two main subsets with distinct patterns of integrin and coreceptor expression. Thus, we identify new cellular components of B cell memory and propose a model for long-term protective immunity that is regulated by a complex balance of committed memory B cells with subspecialized immune function.

subject areas

  • Animals
  • Antigens, CD
  • Antigens, CD45
  • Antigens, CD79
  • B-Lymphocyte Subsets
  • B-Lymphocytes
  • Base Sequence
  • Bone Marrow Cells
  • Cell Differentiation
  • Female
  • Hemocyanin
  • Immunoglobulin lambda-Chains
  • Immunologic Memory
  • Immunophenotyping
  • Macrophage-1 Antigen
  • Membrane Glycoproteins
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Proteoglycans
  • Spleen
  • Syndecan-1
  • Syndecans
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Research

keywords

  • B lymphocyte memory
  • B lymphocyte subset
  • antigen-specific immunity
  • immunological memory
  • mice
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Identity

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.191.7.1149

PubMed ID

  • 10748233
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Additional Document Info

start page

  • 1149

end page

  • 1165

volume

  • 191

issue

  • 7

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