Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Conditional regulation of the human CYP4X1 and CYP4Z1 genes

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Savas, O.
  • Hsu, M. H.
  • Griffin, K. J.
  • Bell, D. R.
  • Johnson, Eric

publication date

  • April 2005

journal

  • Archives of Biochemistry and Biophysics  Journal

abstract

  • Cytochrome P450 genes (CYPs) encoding two new subfamilies designated CYP4X1 and CYP4Z1 were identified in the human genome and the Expressed Sequence Tags database. Partial cDNAs encoding both P450s were isolated from human kidney and used to determine tissue distribution. CYP4X1 was predominantly expressed in trachea and aorta, whereas CYP4Z1 mRNA was preferentially expressed in mammary tissue. In T47-D cells, CYP4Z1 mRNA levels were induced by dexamethasone (14-fold) or by progesterone (10-fold). The induction by these compounds was suppressed by co-treatment with the progesterone and glucocorticoid receptor antagonist mifepristone (RU486). In the progesterone receptor negative MCF-7 cells, CYP4Z1 mRNA was induced by dexamethasone but not by progesterone treatment. CYP4Z1 mRNA levels were unaffected by 17beta-estradiol. In confluent cultures of human hepatoma HepG2 cells that stably express a mouse peroxisome proliferator activated receptor-alpha (PPARalpha) mutant, CYP4X1 mRNA was undetectable in vehicle-treated cells but was readily detectable following addition of the PPARalpha agonist Wy14643. This suggests that PPARalpha activation can affect human CYP4X1 gene transcription. These results demonstrate selective tissue expression and implicate PPARalpha in CYP4X1 regulation, and the glucocorticoid and progesterone receptors in CYP4Z1 gene activation.

subject areas

  • Animals
  • Aorta
  • Breast
  • COS Cells
  • Cell Line, Tumor
  • Cloning, Molecular
  • Cytochrome P-450 Enzyme System
  • DNA Restriction Enzymes
  • DNA, Complementary
  • Databases as Topic
  • Dexamethasone
  • Expressed Sequence Tags
  • Gene Expression Regulation
  • Humans
  • Immunoblotting
  • Isoenzymes
  • Mifepristone
  • Polymerase Chain Reaction
  • Progesterone
  • Pyrimidines
  • RNA
  • RNA, Messenger
  • Receptors, Glucocorticoid
  • Receptors, Progesterone
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tissue Distribution
  • Trachea
  • Transcription, Genetic
  • Transcriptional Activation
scroll to property group menus

Research

keywords

  • HepG2
  • MCF-7
  • PPAR alpha
  • T47-D
  • aorta
  • breast
  • dexamethasone
  • gene regulation
  • human CYP4X1
  • human CYP4Z1
  • progesterone
  • trachea
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0003-9861

Digital Object Identifier (DOI)

  • 10.1016/j.abb.2005.02.022

PubMed ID

  • 15797250
scroll to property group menus

Additional Document Info

start page

  • 377

end page

  • 385

volume

  • 436

issue

  • 2

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support