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The selection of s-cerevisiae mutants defective in the start event of cell-division

Academic Article
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Overview

authors

  • Reed, Steven

publication date

  • 1980

journal

  • Genetics  Journal

abstract

  • Thirty-three temperature-sensitive mutations defective in the start event of the cell division cycle of Saccharomyces cerevisiae were isolated and subjected to preliminary characterization. Complementation studies assigned thes mutations to four complementation groups, one of which, cdc28, has been described previously. Genetic analysis revealed that these complementation groups define single nuclear genes, unlinked to one another. One of the three newly identified genes, cdc37, has been located in the yeast linkage map on chromosome IV, two meiotic map units distal to hom2.--Each mutation produces stage-specific arrest of cell division at start, the same point where mating pheromone interrupts division. After synchronization at start by incubation at the restrictive temperature, the mutants retain the capacity to enlarge and to conjugate.

subject areas

  • Cell Division
  • Chromosome Mapping
  • Genes
  • Genetic Complementation Test
  • Genetic Linkage
  • Mutation
  • Phenotype
  • Saccharomyces cerevisiae
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Identity

PubMed Central ID

  • PMC1214247

International Standard Serial Number (ISSN)

  • 0016-6731

PubMed ID

  • 7002718
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Additional Document Info

start page

  • 561

end page

  • 577

volume

  • 95

issue

  • 3

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