Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Microbial glycosyltransferases for carbohydrate synthesis: Alpha-2,3-sialyltransferase from neisseria gonorrheae

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Izumi, M.
  • Shen, G. J.
  • Wacowich-Sgarbi, S.
  • Nakatani, T.
  • Plettenburg, O.
  • Wong, Chi-Huey

publication date

  • 2001

journal

  • Journal of the American Chemical Society  Journal

abstract

  • The alpha-2,3-sialyltransferase from Neisseria gonorrheae was overproduced in E. coli for exploitation of its substrate specificity and synthetic utility. Several potential acceptor substrates were synthesized in this study, including mono- and oligosaccharides, glycolipids, and glycopeptides and their sulfate derivatives. Some CMP-sialic acid derivatives with modification at the C-5 position were also prepared for evaluation as donor substrates. It was found that the enzyme exhibits a broader acceptor substrate specificity when compared to other sialyltransferases, though the donor specificity is quite limited. Application of the enzyme to the preparative synthesis of representative sialyl glycoconjugates has been demonstrated. On the basis of this work and the work of others, this enzyme is the most versatile and synthetically useful among all sialyltransferases known to date, especially for the synthesis of sulfate-containing glycoconjugates.

subject areas

  • Carbohydrates
  • Cytidine Monophosphate N-Acetylneuraminic Acid
  • N-Acetylneuraminic Acid
  • Neisseria gonorrhoeae
  • Sialyltransferases
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja011382r

PubMed ID

  • 11686694
scroll to property group menus

Additional Document Info

start page

  • 10909

end page

  • 10918

volume

  • 123

issue

  • 44

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support