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Microfluidic serial dilution circuit

Academic Article
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Overview

authors

  • Paegel, Brian
  • Grover, W. H.
  • Skelley, A. M.
  • Mathies, R. A.
  • Joyce, Gerald

publication date

  • November 2006

journal

  • Analytical Chemistry  Journal

abstract

  • In vitro evolution of RNA molecules requires a method for executing many consecutive serial dilutions. To solve this problem, a microfluidic circuit has been fabricated in a three-layer glass-PDMS-glass device. The 400-nL serial dilution circuit contains five integrated membrane valves: three two-way valves arranged in a loop to drive cyclic mixing of the diluent and carryover, and two bus valves to control fluidic access to the circuit through input and output channels. By varying the valve placement in the circuit, carryover fractions from 0.04 to 0.2 were obtained. Each dilution process, which is composed of a diluent flush cycle followed by a mixing cycle, is carried out with no pipeting, and a sample volume of 400 nL is sufficient for conducting an arbitrary number of serial dilutions. Mixing is precisely controlled by changing the cyclic pumping rate, with a minimum mixing time of 22 s. This microfluidic circuit is generally applicable for integrating automated serial dilution and sample preparation in almost any microfluidic architecture.

subject areas

  • Dimethylpolysiloxanes
  • Glass
  • Microfluidics
  • Silicones
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Identity

PubMed Central ID

  • PMC2566538

International Standard Serial Number (ISSN)

  • 0003-2700

Digital Object Identifier (DOI)

  • 10.1021/ac0608265

PubMed ID

  • 17073422
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Additional Document Info

start page

  • 7522

end page

  • 7527

volume

  • 78

issue

  • 21

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