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Constraining G1-specific transcription to late G1 phase: the MBF-associated corepressor Nrm1 acts via negative feedback

Academic Article
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Overview

authors

  • de Bruin, R. A. M.
  • Kalashnikova, T. I.
  • Chahwan, C.
  • McDonald, W. H.
  • Wohlschlegel, J.
  • Yates III, John
  • Russell, Paul
  • Wittenberg, Curt

publication date

  • August 2006

journal

  • Molecular Cell  Journal

abstract

  • G1-specific transcription in yeast depends upon SBF and MBF. We have identified Nrm1 (negative regulator of MBF targets 1), as a stable component of MBF. NRM1 (YNR009w), an MBF-regulated gene expressed during late G1 phase, associates with G1-specific promoters via MBF. Transcriptional repression upon exit from G1 phase requires both Nrm1 and MBF. Inactivation of Nrm1 results in prolonged expression of MBF-regulated transcripts and leads to hydroxyurea (HU) resistance and enhanced bypass of rad53Delta- and mec1Delta-associated lethality. Constitutive expression of a stabilized form of Nrm1 represses MBF targets and leads to HU sensitivity. The fission yeast homolog SpNrm1, encoded by the MBF target gene nrm1(+) (SPBC16A3.07c), binds to MBF target genes and acts as a corepressor. In both yeasts, MBF represses G1-specific transcription outside of G1 phase. A negative feedback loop involving Nrm1 bound to MBF leads to transcriptional repression as cells exit G1 phase.

subject areas

  • Amino Acid Sequence
  • Cell Cycle Proteins
  • Checkpoint Kinase 2
  • Feedback, Physiological
  • G1 Phase
  • Gene Expression Regulation, Fungal
  • Genes, Fungal
  • Hydroxyurea
  • Intracellular Signaling Peptides and Proteins
  • Models, Genetic
  • Molecular Sequence Data
  • Mutation
  • Promoter Regions, Genetic
  • Protein Binding
  • Protein-Serine-Threonine Kinases
  • RNA, Messenger
  • Repressor Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces
  • Trans-Activators
  • Transcription Factors
  • Transcription, Genetic
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Identity

International Standard Serial Number (ISSN)

  • 1097-2765

Digital Object Identifier (DOI)

  • 10.1016/j.molcel.2006.06.025

PubMed ID

  • 16916637
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Additional Document Info

start page

  • 483

end page

  • 496

volume

  • 23

issue

  • 4

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