Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Membrane cofactor protein is a receptor for adenoviruses associated with epidemic keratoconjunctivitis

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

related to degree

  • Wu, Eugene, Ph.D. in Biology, Scripps Research 1998 - 2003

authors

  • Wu, Eugene
  • Trauger, S. A.
  • Pache, L.
  • Mullen, T. M.
  • Von Seggern, D. J.
  • Siuzdak, Gary
  • Nemerow, Glen

publication date

  • April 2004

journal

  • Journal of Virology  Journal

abstract

  • Subgroup D adenovirus (Ad) types 8, 19, and 37 (Ad8, -19, and -37, respectively) are causative agents of epidemic keratoconjunctivitis and genital tract infections. Previous studies showed that Ad37 binds to a 50-kDa membrane glycoprotein expressed on human ocular (conjunctival) cells. To identify and characterize the role of the 50-kDa glycoprotein in Ad37 infection, we partially purified this molecule from solubilized Chang C conjunctival cell membranes by using lentil lectin chromatography and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Liquid chromatography coupled to nano-electrospray ionization-tandem mass spectrometry was subsequently used to identify four Ad37 receptor candidates: CD46, CD87, CD98, and CD147. Immunodepletion analyses demonstrated that the 50-kDa protein is identical to CD46 (also known as membrane cofactor protein). The Ad37, but not Ad5, fiber knob bound to the extracellular domain of CD46, demonstrating a direct interaction of an Ad37 capsid protein with CD46. An antibody specific for the N-terminal 19 amino acids of CD46 also blocked Ad37 infection of human cervical carcinoma and conjunctival cells, indicating a requirement for CD46 in infection. Finally, expression of a 50-kDa isoform of human CD46 in a CD46-null cell line increased cell binding by wild-type Ad37 and gene delivery by an Ad vector pseudotyped with the Ad37 fiber, but not by a vector bearing the Ad5 fiber. Together, these studies demonstrate that CD46 serves as an attachment receptor for Ad37 and shed further light on the cell entry pathway of subgroup D Ads.

subject areas

  • Adenoviridae Infections
  • Adenoviruses, Human
  • Amino Acid Sequence
  • Animals
  • Antigens, CD
  • Antigens, CD46
  • Base Sequence
  • CHO Cells
  • Cell Line
  • Cricetinae
  • DNA, Viral
  • HeLa Cells
  • Humans
  • Keratoconjunctivitis
  • Membrane Glycoproteins
  • Molecular Sequence Data
  • Receptors, Virus
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.78.8.3897-3905.2004

PubMed ID

  • 15047806
scroll to property group menus

Additional Document Info

start page

  • 3897

end page

  • 3905

volume

  • 78

issue

  • 8

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support