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In vitro studies of the genetically determined unresponsiveness to thymus-independent antigens in CBA/N mice

Academic Article
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Overview

authors

  • Cohen, P. L.
  • Scher, I.
  • Mosier, Donald

publication date

  • 1976

journal

  • Journal of Immunology  Journal

abstract

  • The X-chromosome-linked B lymphocyte defect of CBA/N mice has been studied in vitro by comparing the ability of (CBA/N X DBA/2)F1 (X-/X- X X+/Y) male (X-/Y) and female (X-/X+) spleen cells to respond to the thymus-independent antigen DNP (or TNP)-AECM-Ficoll. (CBA/N X DBA/2)F1 male spleen cells failed to generate significant in vitro anti-TNP antibody responses to DNP- or TNP-AECM-Ficoll, in contrast to spleen cells from F1 female (X-/X+) mice which responded normally to these T-independent antigens. Spleen cells from male F1 mice responded almost as well as F1 female cells to the thymus-dependent antigen, TNP-sheep red blood cells (TNP-SRBC) in vitro. Adding F1 male cells to F1 female cells failed to reduce the response of the latter to DNP-AECM-Ficoll, suggesting that the inability of F1 male cells to respond was not due to active suppression. The response of F1 male spleen cells to TNP-SRBC was not impaired by adding high concentrations of TNP-AECM-Ficoll indicating that the mechanism of unresponsiveness was not tolerance induction in all TNP-specific precursors. Lymphocytes from F1 male mice were capable of forming anti-TNP antibody after stimulation with lipopolysaccharide (LPS) in high concentrations; DNP-AECM-Ficoll had no effect on this polyclonal response. B lymphocytes from mice bearing only the X-chromosome of the CBA/N strain thus display a profound defect in B cell activation. This functional defect may represent either an inability of the defective B cells to be activated by thymus-independent antigens or the absence of a sub-class of B cells which respond to thymus-independent antigens.

subject areas

  • Animals
  • Antibody Formation
  • Antigens
  • Epitopes
  • Female
  • Ficoll
  • Immunosuppression
  • In Vitro Techniques
  • Lymphocyte Activation
  • Male
  • Methylurea Compounds
  • Mice
  • Mice, Inbred CBA
  • Mice, Inbred DBA
  • Nitrobenzenes
  • Polysaccharides, Bacterial
  • Sex Factors
  • Spleen
  • Thymus Gland
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 55435
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Additional Document Info

start page

  • 301

end page

  • 304

volume

  • 116

issue

  • 2

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