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Visualization of the genesis and fate of isotype-switched b cells during a primary immune response

Academic Article
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Overview

authors

  • Pape, K. A.
  • Kouskoff, V.
  • Nemazee, David
  • Tang, H. L.
  • Cyster, J. G.
  • Tze, L. E.
  • Hippen, K. L.
  • Behrens, T. W.
  • Jenkins, M. K.

publication date

  • June 2003

journal

  • Journal of Experimental Medicine  Journal

abstract

  • The life history of isotype-switched B cells is unclear, in part, because of an inability to detect rare antigen-specific B cells at early times during the immune response. To address this issue, a small population of B cells carrying targeted antibody transgenes capable of class switching was monitored in immunized mice. After contacting helper T cells, the first switched B cells appeared in follicles rather than in the red pulp, as was expected. Later, some of the switched B cells transiently occupied the red pulp and marginal zone, whereas others persisted in germinal centers (GCs). Antigen-experienced IgM B cells were rarely found in GCs, indicating that these cells switched rapidly after entering GCs or did not persist in this environment.

subject areas

  • Adoptive Transfer
  • Animals
  • Antigens
  • B-Lymphocytes
  • Humans
  • Immunoglobulin Class Switching
  • Immunoglobulin M
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Muramidase
  • Ovalbumin
  • Phenotype
  • Spleen
  • T-Lymphocytes, Helper-Inducer
  • Transgenes
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Research

keywords

  • T cells
  • germinal centers
  • lymphocyte activation
  • marginal zone
  • transgenic mice
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Identity

PubMed Central ID

  • PMC2193962

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.20012065

PubMed ID

  • 12796466
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Additional Document Info

start page

  • 1677

end page

  • 1687

volume

  • 197

issue

  • 12

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