Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

High-performance liquid chromatographic determination of D-amino acid oxidase activity

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Biondi, P. A.
  • Guidotti, Luca
  • Negri, A.
  • Secchi, C.

publication date

  • May 1991

journal

  • Journal of Chromatography-Biomedical Applications  Journal

abstract

  • A new procedure for the assay of D-amino acid oxidase activity has been developed. alpha-Ketoisovaleric acid, derived from D-valine, was estimated by high-performance liquid chromatography after reaction with o-phenylenediamine to give the corresponding quinoxalinol derivative. alpha-Ketovaleric acid was used as an internal standard to ensure the reproducibility of the method. As an example of application, kidney cortex homogenates were analyzed for their D-amino acid oxidase activity. The advantages of the presented procedure for the determination of the enzymatic activity in biological samples compared with previously reported procedures are discussed.

subject areas

  • Animals
  • Cattle
  • Chromatography, High Pressure Liquid
  • D-Amino-Acid Oxidase
  • Keto Acids
  • Kidney
  • Pentanoic Acids
  • Phenylenediamines
  • Swine
  • Valerates
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0378-4347

Digital Object Identifier (DOI)

  • 10.1016/0378-4347(91)80254-a

PubMed ID

  • 1682335
scroll to property group menus

Additional Document Info

start page

  • 377

end page

  • 382

volume

  • 566

issue

  • 2

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support