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Natural-like replication of an unnatural base pair for the expansion of the genetic alphabet and biotechnology applications

Academic Article
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Overview

related to degree

  • Malyshev, Denis A, Ph.D. in Chemical Biology, Scripps Research 2008 - 2013

authors

  • Li, L.
  • Degardin, M.
  • Lavergne, T.
  • Malyshev, Denis A
  • Dhami, K.
  • Ordoukhanian, Phillip
  • Romesberg, Floyd

publication date

  • January 2014

journal

  • Journal of the American Chemical Society  Journal

abstract

  • We synthesized a panel of unnatural base pairs whose pairing depends on hydrophobic and packing forces and identify dTPT3-dNaM, which is PCR amplified with a natural base pair-like efficiency and fidelity. In addition, the dTPT3 scaffold is uniquely tolerant of attaching a propargyl amine linker, resulting in the dTPT3(PA)-dNaM pair, which is amplified only slightly less well. The identification of dTPT3 represents significant progress toward developing an unnatural base pair for the in vivo expansion of an organism's genetic alphabet and for a variety of in vitro biotechnology applications where it is used to site-specifically label amplified DNA, and it also demonstrates for the first time that hydrophobic and packing forces are sufficient to mediate natural-like replication.

subject areas

  • Base Pairing
  • Biomimetic Materials
  • Biotechnology
  • Hydrophobic and Hydrophilic Interactions
  • Kinetics
  • Polymerase Chain Reaction
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Identity

PubMed Central ID

  • PMC3979842

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja408814g

PubMed ID

  • 24152106
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Additional Document Info

start page

  • 826

end page

  • 829

volume

  • 136

issue

  • 3

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