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Plasmalemmal insertion and modification of sodium channels at the nerve growth cone

Academic Article
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Overview

authors

  • Wood, Malcolm R.
  • Debin, J.
  • Strichartz, G. R.
  • Pfenninger, K. H.

publication date

  • August 1992

journal

  • Journal of Neuroscience  Journal

abstract

  • We have characterized voltage-dependent sodium channels in growth cones (GCPs) isolated from fetal rat brain using saxitoxin and TTX binding as well as recordings from channels reconstituted into lipid bilayer membranes. Both high- and low-affinity binding sites are present in GCP membranes. However, the two binding sites are segregated largely or completely, with the high-affinity binding sites in the plasmalemma, and the low-affinity sites in an internal membrane compartment. Plasmalemmal insertion of these internal sites can be triggered by high-potassium depolarization and depends on a metalloendoprotease-requiring mechanism. These observations indicate that a precursor-product relationship exists between the internal and external sodium channels of the growth cone, and therefore suggest that channel externalization causes conversion of low-affinity to high-affinity saxitoxin receptors. This conversion may represent a step of channel capacitation.

subject areas

  • Animals
  • Binding Sites
  • Brain
  • Cell Membrane
  • Fetus
  • Lipid Bilayers
  • Protease Inhibitors
  • Rats
  • Saxitoxin
  • Sodium Channels
  • Tetrodotoxin
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Identity

International Standard Serial Number (ISSN)

  • 0270-6474

PubMed ID

  • 1322979
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Additional Document Info

start page

  • 2948

end page

  • 2959

volume

  • 12

issue

  • 8

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