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A chloride channel reconstituted from fetal rat brain growth cones

Academic Article
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Overview

authors

  • Debin, J. A.
  • Wood, Malcolm R.
  • Pfenninger, K. H.
  • Strichartz, G. R.

publication date

  • July 1994

journal

  • Journal of Membrane Biology  Journal

abstract

  • Chloride channels were reconstituted into planar lipid bilayers isolated from a preparation of growth cone particles (GCPs) isolated from fetal rat brain. One type of channel was predominantly seen and some of its biophysical and pharmacological properties were studied. The single channel i-V relationship was curvilinear with a chord conductance of 75 pS at +30 mV in symmetric 200 mM NaCl solutions buffered with phosphate. The channel was inactivated by depolarization, and this inactivation was reversed rapidly upon returning to -25 mV. The Cl- channel was significantly permeant to Na+ ions (PNa/PCl = 0.26), and the relative halide permeabilities were determined to be: I(1.92) > Br(1.73) > Cl(1.0) > F(0.34). The channel was inhibited by the common stilbene compounds (DIDS, SITS, DNDS), as well as by Zn2+ ions and an indanyloxyacetic acid derivative. A developmental role for the GCP Cl- channel is suggested by the observation that adult rat brain synaptosomal membranes were nearly devoid of this type of Cl- channel.

subject areas

  • 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
  • 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
  • Animals
  • Brain
  • Cell Membrane
  • Chloride Channels
  • Membranes, Artificial
  • Rats
  • Retinal Cone Photoreceptor Cells
  • Stilbenes
  • Synaptosomes
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Research

keywords

  • ANION CHANNEL
  • CHLORIDE CHANNEL
  • DIDS
  • DNDS
  • GROWTH CONE
  • SITS
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Identity

International Standard Serial Number (ISSN)

  • 0022-2631

PubMed ID

  • 7966248
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Additional Document Info

start page

  • 7

end page

  • 19

volume

  • 141

issue

  • 1

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