Genomically recoded organisms expand biological functions

Academic Article

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Overview

authors

• Lajoie, M. J.
• Rovner, A. J.
• Goodman, D. B.
• Aerni, H. R.
• Haimovich, A. D.
• Kuznetsov, G.
• Mercer, J. A.
• Wang, H. H.
• Carr, P. A.
• Mosberg, J. A.
• Rohland, N.
• Schultz, Peter
• Jacobson, J. M.
• Rinehart, J.
• Church, G. M.
• Isaacs, F. J.

publication date

• 2013

journal

• Science  Journal

abstract

• We describe the construction and characterization of a genomically recoded organism (GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 with synonymous UAA codons, which permitted the deletion of release factor 1 and reassignment of UAG translation function. This GRO exhibited improved properties for incorporation of nonstandard amino acids that expand the chemical diversity of proteins in vivo. The GRO also exhibited increased resistance to T7 bacteriophage, demonstrating that new genetic codes could enable increased viral resistance.

subject areas

• Amino Acid Substitution
• Amino Acids
• Bacteriophage T7
• Codon, Terminator
• Escherichia coli
• Escherichia coli Proteins
• Genetic Engineering
• Genome, Bacterial
• Organisms, Genetically Modified
• Peptide Chain Termination, Translational
• Peptide Termination Factors

Identity

PubMed Central ID

• PMC4924538

International Standard Serial Number (ISSN)

• 0036-8075

Digital Object Identifier (DOI)

• 10.1126/science.1241459

PubMed ID

• 24136966

Additional Document Info

start page

• 357

end page

• 360

volume

• 342

issue

• 6156