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Natural killer T (NKT)-B-cell interactions promote prolonged antibody responses and long-term memory to pneumococcal capsular polysaccharides

Academic Article
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Overview

authors

  • Bai, L.
  • Deng, S.
  • Reboulet, R.
  • Mathew, R.
  • Teyton, Luc
  • Savage, P. B.
  • Bendelac, A.

publication date

  • October 2013

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Innate-like natural killer T (NKT) cells critically enhance cell and humoral immunity against infections through recognition of conserved microbial lipid antigens presented by CD1d-expressing antigen-presenting cells, and provision of CD40L and cytokine signals. Whereas NKT cells efficiently licensed dendritic cells to prime potent effector and memory T cells, studies based on model antigens such as alphagalactosylceramide-nitrophenyl conjugates concluded that help to B cells was associated with NKT follicular helper differentiation, but limited to short-term responses without induction of memory. We revisited this surprising conclusion in the context of the extracellular encapsulated pathogen Streptococcus pneumoniae, where recognition of lipid and capsular polysaccharide antigens by NKT cells and B cells, respectively, provide critical host protection. Using liposomal nanoparticles displaying synthetic lipid and polysaccharide antigens to elicit pure and direct NKT-B-cell interactions in vivo, we observed intense and prolonged antibody responses with isotype switch, affinity maturation, and long-lasting B-cell memory, despite modest or absent NKT follicular helper differentiation. Furthermore, conditional ablation of Cd1d demonstrated a requirement for a two-step process involving first cognate interactions with dendritic cells, for NKT cell activation, and then with B cells, for induction of isotype switch and memory. Thus, NKT help to B cells represents both a major arm of antimicrobial defense and a promising target for B-cell vaccines.

subject areas

  • Animals
  • Antibodies
  • B-Lymphocytes
  • Cell Communication
  • Cell Differentiation
  • Flow Cytometry
  • Immunologic Memory
  • Mice
  • Mice, Inbred C57BL
  • Nanoparticles
  • Natural Killer T-Cells
  • Pneumococcal Infections
  • Polysaccharides, Bacterial
  • Statistics, Nonparametric
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Identity

PubMed Central ID

  • PMC3791701

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.1303218110

PubMed ID

  • 24043771
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Additional Document Info

start page

  • 16097

end page

  • 16102

volume

  • 110

issue

  • 40

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