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Copresentation of antigen and ligands of Siglec-G induces B cell tolerance independent of CD22

Academic Article
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Overview

authors

  • Pfrengle, F.
  • Macauley, Matthew
  • Kawasaki, N.
  • Paulson, James

publication date

  • August 2013

journal

  • Journal of Immunology  Journal

abstract

  • Differentiation of self from nonself is indispensable for maintaining B cell tolerance in peripheral tissues. CD22 and Siglec-G (sialic acid-binding Ig-like lectin G) are two inhibitory coreceptors of the BCR that are implicated in maintenance of tolerance to self Ags. Enforced ligation of CD22 and the BCR by a nanoparticle displaying both Ag and CD22 ligands induces a tolerogenic circuit resulting in apoptosis of the Ag-reactive B cell. Whether Siglec-G also has this property has not been investigated in large part owing to the lack of a selective Siglec-G ligand. In this article, we report the development of a selective high-affinity ligand for Siglec-G and its application as a chemical tool to investigate the tolerogenic potential of Siglec-G. We find that liposomal nanoparticles decorated with Ag and Siglec-G ligand inhibit BCR signaling in both B1 and B2 B cells compared with liposomes displaying Ag alone. Not only is inhibition of B cell activation observed by ligating the BCR with Siglec-G, but robust tolerance toward T-independent and T-dependent Ags is also induced in mice. The ability of Siglec-G to inhibit B cell activation equally in both B1 and B2 subsets is consistent with our observation that Siglec-G is expressed at a relatively constant level throughout numerous B cell subsets. These results suggest that Siglec-G may contribute to maintenance of B cell tolerance toward self Ags in various B cell compartments.

subject areas

  • Animals
  • Antibodies, Monoclonal
  • B-Lymphocyte Subsets
  • Calcium Signaling
  • Dendritic Cells
  • Gene Expression Regulation
  • Germinal Center
  • Immune Tolerance
  • Immunologic Memory
  • Lectins
  • Ligands
  • Liposomes
  • Lymphopoiesis
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Mice, Transgenic
  • Organ Specificity
  • Protein Tyrosine Phosphatase, Non-Receptor Type 6
  • Receptors, Antigen, B-Cell
  • Sialic Acid Binding Ig-like Lectin 2
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Identity

PubMed Central ID

  • PMC3735655

International Standard Serial Number (ISSN)

  • 0022-1767

Digital Object Identifier (DOI)

  • 10.4049/jimmunol.1300921

PubMed ID

  • 23836061
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Additional Document Info

start page

  • 1724

end page

  • 1731

volume

  • 191

issue

  • 4

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