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Influences on Trimerization and Aggregation of Soluble, Cleaved HIV-1 SOSIP Envelope Glycoprotein

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Overview

related to degree

  • Lee, Jeong Hyun, Ph.D. in Chemical Biology, Scripps Research 2011 - 2016

authors

  • Klasse, P. J.
  • Depetris, R. S.
  • Pejchal, R.
  • Julien, J. P.
  • Khayat, R.
  • Lee, Jeong Hyun
  • Marozsan, A. J.
  • Cupo, A.
  • Cocco, N.
  • Korzun, J.
  • Yasmeen, A.
  • Ward, Andrew
  • Wilson, Ian
  • Sanders, R. W.
  • Moore, J. P.

publication date

  • September 2013

journal

  • Journal of Virology  Journal

abstract

  • We describe methods to improve the properties of soluble, cleaved gp140 trimers of the human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (Env) for use in structural studies and as immunogens. In the absence of nonionic detergents, gp140 of the KNH1144 genotype, terminating at residue 681 in gp41 (SOSIP.681), has a tendency to form higher-order complexes or aggregates, which is particularly undesirable for structure-based research. We found that this aggregation in the absence of detergent does not involve the V1, V2, or V3 variable regions of gp120. Moreover, we observed that detergent forms micelles around the membrane-proximal external region (MPER) of the SOSIP.681 gp140 trimers, whereas deletion of most of the MPER residues by terminating the gp140 at residue 664 (SOSIP.664) prevented the aggregation that otherwise occurs in SOSIP.681 in the absence of detergent. Although the MPER can contribute to trimer formation, truncation of most of it only modestly reduced trimerization and lacked global adverse effects on antigenicity. Thus, the MPER deletion minimally influenced the kinetics of the binding of soluble CD4 and a CD4-binding site antibody to immobilized trimers, as detected by surface plasmon resonance. Furthermore, the MPER deletion did not alter the overall three-dimensional structure of the trimers, as viewed by negative-stain electron microscopy. Homogeneous and aggregate-free MPER-truncated SOSIP Env trimers are therefore useful for immunogenicity and structural studies.

subject areas

  • Amino Acid Sequence
  • Antibodies, Monoclonal
  • Antibodies, Neutralizing
  • Antigens, CD4
  • Binding Sites
  • Detergents
  • HEK293 Cells
  • HIV Antibodies
  • HIV Envelope Protein gp120
  • HIV Envelope Protein gp41
  • HIV-1
  • Humans
  • Microscopy, Electron
  • Molecular Sequence Data
  • Protein Multimerization
  • Protein Structure, Quaternary
  • Recombinant Proteins
  • Sequence Deletion
  • Solubility
  • env Gene Products, Human Immunodeficiency Virus
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Identity

PubMed Central ID

  • PMC3754145

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.01226-13

PubMed ID

  • 23824824
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Additional Document Info

start page

  • 9873

end page

  • 9885

volume

  • 87

issue

  • 17

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