Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Bone marrow plasma cells are a primary source of serum HIV-1-specific antibodies in chronically infected individuals

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Montezuma-Rusca, J. M.
  • Moir, S.
  • Kardava, L.
  • Buckner, C. M.
  • Louie, A.
  • Kim, L. J. Y.
  • Santich, B. H.
  • Wang, W.
  • Fankuchen, O. R.
  • Diaz, G.
  • Daub, J. R.
  • Rosenzweig, S. D.
  • Chun, T. W.
  • Li, Yuxing
  • Braylan, R. C.
  • Calvo, K. R.
  • Fauci, A. S.

publication date

  • March 2015

journal

  • Journal of Immunology  Journal

abstract

  • Several potent and broadly neutralizing Abs to HIV-1 have been isolated recently from peripheral blood B cells of infected individuals, based on prescreening of Ab activity in the serum. However, little is known regarding the cells that make the Abs that circulate in the blood. Accordingly, we investigated the most likely source, the bone marrow, of chronically HIV-1-infected individuals who were not receiving antiretroviral therapy. Increased frequencies of plasma cells, as well as B cell precursors, namely preB-I and preB-II, and decreased frequencies of mature B cells were observed in bone marrow aspirates of these individuals compared with HIV-negative counterparts. Increased frequencies of bone marrow plasma cells are consistent with known hallmarks of HIV-1 infection, namely hypergammaglobulinemia and increased frequencies of peripheral blood plasmablasts. Levels of HIV-1 envelope (Env)-binding and HIV-1-neutralizing Abs were measured in serum, and corresponding frequencies of Ab-secreting or Env-binding cells were measured in the blood (plasmablasts and memory B cells) and in the bone marrow (plasma cells). A strong correlation was observed between serum HIV-1-specific Abs and Env-specific bone marrow-derived plasma cells, but not circulating plasmablasts or memory B cells. These findings demonstrate that, despite HIV-1-induced phenotypic and functional B cell dysregulation in the peripheral blood and secondary lymphoid tissues, bone marrow plasma cells remain a primary source for circulating HIV-1-specific Abs in HIV-1-infected individuals.

subject areas

  • Adult
  • Antibodies, Neutralizing
  • B-Lymphocyte Subsets
  • B-Lymphocytes
  • Bone Marrow
  • Bone Marrow Cells
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Flow Cytometry
  • HIV Antibodies
  • HIV Infections
  • HIV-1
  • Host-Pathogen Interactions
  • Humans
  • Immunologic Memory
  • Lymphocyte Count
  • Male
  • Plasma Cells
  • Precursor Cells, B-Lymphoid
  • Young Adult
  • env Gene Products, Human Immunodeficiency Virus
scroll to property group menus

Identity

PubMed Central ID

  • PMC4355319

International Standard Serial Number (ISSN)

  • 0022-1767

Digital Object Identifier (DOI)

  • 10.4049/jimmunol.1402424

PubMed ID

  • 25681347
scroll to property group menus

Additional Document Info

start page

  • 2561

end page

  • 2568

volume

  • 194

issue

  • 6

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support