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Smoke-derived karrikin perception by the α/β-hydrolase KAI2 from Arabidopsis

Academic Article
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Overview

authors

  • Guo, Y.
  • Zheng, Z.
  • La Clair, J. J.
  • Chory, Joanne
  • Noel, J. P.

publication date

  • May 2013

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Genetic studies in Arabidopsis implicate an α/β-hydrolase, KARRIKIN-INSENSITIVE 2 (KAI2) as a receptor for karrikins, germination-promoting butenolide small molecules found in the smoke of burned plants. However, direct biochemical evidence for the interaction between KAI2 and karrikin and for the mechanism of downstream signaling by a KAI2-karrikin complex remain elusive. We report crystallographic analyses and ligand-binding experiments for KAI2 recognition of karrikins. The karrikin-1 (KAR1) ligand sits in the opening to the active site abutting a helical domain insert but distal from the canonical catalytic triad (Ser95-His246-Asp217) of α/β-hydrolases, consistent with the lack of detectable hydrolytic activity by purified KAI2. The closest approach of KAR1 to Ser95-His246-Asp217 is 3.8 Å from His246. Six aromatic side chains, including His246, encapsulate KAR1 through geometrically defined aromatic-aromatic interactions. KAR1 binding induces a conformational change in KAI2 at the active site entrance. A crevice of hydrophobic residues linking the polar edge of KAR1 and the helical domain insert suggests that KAI2-KAR1 creates a contiguous interface for binding signaling partners in a ligand-dependent manner.

subject areas

  • Arabidopsis
  • Arabidopsis Proteins
  • Catalytic Domain
  • Crystallography, X-Ray
  • Escherichia coli
  • Fires
  • Furans
  • Hydrolases
  • Hydrolysis
  • Hydrophobic and Hydrophilic Interactions
  • Ligands
  • Magnetic Resonance Spectroscopy
  • Models, Molecular
  • Protein Binding
  • Protein Structure, Tertiary
  • Pyrans
  • Seeds
  • Signal Transduction
  • Smoke
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Research

keywords

  • plant signaling
  • seed dormancy
  • wildfires
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Identity

PubMed Central ID

  • PMC3657771

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.1306265110

PubMed ID

  • 23613584
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Additional Document Info

start page

  • 8284

end page

  • 8289

volume

  • 110

issue

  • 20

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