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MYCNOS functions as an antisense RNA regulating MYCN

Academic Article
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Overview

authors

  • Vadie, N.
  • Saayman, S.
  • Lenox, A.
  • Ackley, A.
  • Clemson, M.
  • Burdach, J.
  • Hart, J.
  • Vogt, Peter K.
  • Morris, Kevin

publication date

  • August 2015

journal

  • RNA Biology  Journal

abstract

  • Amplification or overexpression of neuronal MYC (MYCN) is associated with poor prognosis of human neuroblastoma. Three isoforms of the MYCN protein have been described as well as a protein encoded by an antisense transcript (MYCNOS) that originates from the opposite strand at the MYCN locus. Recent findings suggest that some antisense long non-coding RNAs (lncRNAs) can play a role in epigenetically regulating gene expression. Here we report that MYCNOS transcripts function as a modulator of the MYCN locus, affecting MYCN promoter usage and recruiting various proteins, including the Ras GTPase-activating protein-binding protein G3BP1, to the upstream MYCN promoter. Overexpression of MYCNOS results in a reduction of upstream MYCN promoter usage and increased MYCN expression, suggesting that the protein-coding MYCNOS also functions as a regulator of MYCN ultimately controlling MYCN transcriptional variants. The observations presented here demonstrate that protein-coding transcripts can regulate gene transcription and can tether regulatory proteins to target loci.

subject areas

  • Azacitidine
  • Carrier Proteins
  • Cell Line, Tumor
  • Enzyme Inhibitors
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Hydroxamic Acids
  • Models, Genetic
  • Neuroblastoma
  • Nuclear Proteins
  • Oncogene Proteins
  • Promoter Regions, Genetic
  • Protein Isoforms
  • Protein Synthesis Inhibitors
  • RNA, Antisense
  • Reverse Transcriptase Polymerase Chain Reaction
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Research

keywords

  • G3BP1
  • MAP4
  • RNA
  • Transcriptional regulation
  • bidirectional promoter
  • neuroblastoma
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Identity

PubMed Central ID

  • PMC4615167

International Standard Serial Number (ISSN)

  • 1547-6286

Digital Object Identifier (DOI)

  • 10.1080/15476286.2015.1063773

PubMed ID

  • 26156430
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Additional Document Info

start page

  • 893

end page

  • 899

volume

  • 12

issue

  • 8

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