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Just add water: cannabinoid discrimination in a water T-maze with FAAH((-/-)) and FAAH((+/+)) mice

Academic Article
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Overview

authors

  • Wiley, J. L.
  • Lefever, T. W.
  • Pulley, N. S.
  • Marusich, J. A.
  • Cravatt, Benjamin
  • Lichtman, A. H.

publication date

  • August 2016

journal

  • Behavioural Pharmacology  Journal

abstract

  • Incomplete overlap in the discriminative stimulus effects of Δ-tetrahydrocannabinol (THC) and the endocannabinoids, anandamide (AEA) and 2-arachidonoylglycerol has been reported in food-reinforced tasks. The aim of this study was to examine cannabinoid discriminative stimulus effects in a nonappetitive procedure. Adult male mice lacking the gene for AEA's major metabolic enzyme, fatty acid amide hydrolase (FAAH), and FAAH mice were trained to discriminate THC or AEA in a water T-maze, in which the response was swimming to an escape platform on the injection-appropriate side. JZL184, a monoacylglycerol lipase inhibitor, was also tested. FAAH mice showed faster acquisition than FAAH mice. THC and AEA fully substituted, with only minor cross-procedure potency variations. Incomplete substitution of JZL184 was observed in THC-trained FAAH mice in the water-maze task, as contrasted with full substitution in a food-reinforced nose-poke procedure. Stress-induced changes in AEA and/or 2-arachidonoylglycerol concentrations in the brain may have mediated this attenuation. JZL184 also partially substituted in AEA-trained FAAH mice in the water maze, suggesting incomplete overlap in the stimulus effects of AEA and JZL184. Through the use of a novel water-maze procedure, the present study supports the work of previous behavioral pharmacologists in showing the robustness of the discrimination paradigm.
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Research

keywords

  • 2-AG
  • FAAH
  • MAGL
  • anandamide
  • aversion
  • endocannabinoid
  • tetrahydrocannabinol
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Identity

PubMed Central ID

  • PMC4937884

International Standard Serial Number (ISSN)

  • 0955-8810

Digital Object Identifier (DOI)

  • 10.1097/fbp.0000000000000228

PubMed ID

  • 27385208
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Additional Document Info

start page

  • 479

end page

  • 484

volume

  • 27

issue

  • 5

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