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Genomic structure and sequence of the pufferfish (Fugu rubripes) gene encoding an actin-related protein

Academic Article
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Overview

authors

  • Venkatesh, B.
  • Brenner, Sydney

publication date

  • April 1998

journal

  • Gene  Journal

abstract

  • Using a polymerase chain reaction (PCR)-generated gene fragment as a probe, we isolated and sequenced a gene encoding an actin-related protein belonging to the Arp3 family from the pufferfish, Fugu rubripes. The Fugu Arp3 gene spans 3.7kb from the transcription start site to the polyadenylation signal. This is the first report of the genomic sequence of a vertebrate Arp3 gene. The Fugu Arp3 gene consists of 12 exons and 11 introns compared to Drosophila homologue, which has six exons and five introns. The protein sequence encoded by the Fugu gene is 97% and 80% identical to bovine and Drosophila homologues, respectively. The Fugu Arp3 gene is expressed in a wide range of tissues, with higher levels detected in the gills, kidney, ovary, skin and testis. Three different size transcripts of the Arp3 gene (1.4kb, 1.8kb and 2.2kb) were identified in various tissues.

subject areas

  • Actin-Related Protein 3
  • Actins
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Conserved Sequence
  • Cytoskeletal Proteins
  • Fishes
  • Gene Expression Regulation
  • Introns
  • Molecular Sequence Data
  • RNA, Messenger
  • Sequence Alignment
  • Sequence Analysis
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Research

keywords

  • Arp3 gene
  • exon-intron structure
  • expression pattern
  • polymerase chain reaction
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Identity

International Standard Serial Number (ISSN)

  • 0378-1119

Digital Object Identifier (DOI)

  • 10.1016/s0378-1119(98)00096-1

PubMed ID

  • 9573354
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Additional Document Info

start page

  • 169

end page

  • 175

volume

  • 211

issue

  • 1

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