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Collagen-based cell migration models in vitro and in vivo

Academic Article
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Overview

authors

  • Wolf, K.
  • Alexander, S.
  • Schacht, V.
  • Coussens, L. M.
  • von Andrian, U. H.
  • van Rheenen, J.
  • Deryugina, Elena
  • Friedl, P.

publication date

  • October 2009

journal

  • Seminars in Cell & Developmental Biology  Journal

abstract

  • Fibrillar collagen is the most abundant extracellular matrix (ECM) constituent which maintains the structure of most interstitial tissues and organs, including skin, gut, and breast. Density and spatial alignments of the three-dimensional (3D) collagen architecture define mechanical tissue properties, i.e. stiffness and porosity, which guide or oppose cell migration and positioning in different contexts, such as morphogenesis, regeneration, immune response, and cancer progression. To reproduce interstitial cell movement in vitro with high in vivo fidelity, 3D collagen lattices are being reconstituted from extracted collagen monomers, resulting in the re-assembly of a fibrillar meshwork of defined porosity and stiffness. With a focus on tumor invasion studies, we here evaluate different in vitro collagen-based cell invasion models, employing either pepsinized or non-pepsinized collagen extracts, and compare their structure to connective tissue in vivo, including mouse dermis and mammary gland, chick chorioallantoic membrane (CAM), and human dermis. Using confocal reflection and two-photon-excited second harmonic generation (SHG) microscopy, we here show that, depending on the collagen source, in vitro models yield homogeneous fibrillar texture with a quite narrow range of pore size variation, whereas all in vivo scaffolds comprise a range from low- to high-density fibrillar networks and heterogeneous pore sizes within the same tissue. Future in-depth comparison of structure and physical properties between 3D ECM-based models in vitro and in vivo are mandatory to better understand the mechanisms and limits of interstitial cell movements in distinct tissue environments.

subject areas

  • Animals
  • Cell Movement
  • Collagen
  • Extracellular Matrix
  • Humans
  • Models, Biological
  • Neoplasms
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Research

keywords

  • Cancer cell invasion models
  • Confocal reflection microscopy
  • Connective tissue geometry
  • Physical collagen spacing
  • Second harmonic generation microscopy
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Identity

PubMed Central ID

  • PMC4021709

International Standard Serial Number (ISSN)

  • 1084-9521

Digital Object Identifier (DOI)

  • 10.1016/j.semcdb.2009.08.005

PubMed ID

  • 19682592
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Additional Document Info

start page

  • 931

end page

  • 941

volume

  • 20

issue

  • 8

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