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Molecular cloning of the pufferfish (Takifugu rubripes) Mx gene and functional characterization of its promoter

Academic Article
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Overview

authors

  • Yap, W. H.
  • Tay, A.
  • Brenner, Sydney
  • Venkatesh, B.

publication date

  • January 2003

journal

  • Immunogenetics  Journal

abstract

  • Mx proteins are members of a family of interferon-inducible genes that are expressed by cells in response to viral infection. They are important determinants of innate immunity against viral infection in vertebrates. We cloned the pufferfish ( Takifugu rubripes) Mx gene and sequenced 80 kb from the Mx locus. The Fugu Mx gene spans 3.4 kb from the transcription start site to the polyadenylation signal, and is made up of 12 exons and 11 introns. The protein sequence encoded by the Fugu Mx gene is 77%, 48%, and 51% identical to that of trout Mx1, chicken Mx, and mouse Mx1 genes, respectively. The Fugu Mx gene is expressed in a variety of tissues, with high expression detected in the heart, gill, kidney, intestine, and brain. Analysis of the 5'-flanking sequence of the gene showed the presence of two interferon-stimulated response elements (ISRE) at positions -51 to 38 and -97 to 85, relative to the transcription start site. The Fugu Mx promoter was inducible by human IFN-beta in the human hepatoma (Huh7) cells and by polyinosinic: polycytidilic acid in the top minnow hepatoma (PLHC-1) cells. Deletion analysis of the promoter showed that both ISREs contributed to inducibility. These results demonstrate that the molecular mechanisms involved in Mx gene regulation are conserved between fish and mammals.

subject areas

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Chromosome Mapping
  • Cloning, Molecular
  • GTP-Binding Proteins
  • Genetic Structures
  • Guanosine Triphosphate
  • Molecular Sequence Data
  • Myxovirus Resistance Proteins
  • Promoter Regions, Genetic
  • Tetraodontiformes
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Research

keywords

  • Fugu
  • Mx
  • antiviral
  • interferon
  • promoter
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Identity

International Standard Serial Number (ISSN)

  • 0093-7711

Digital Object Identifier (DOI)

  • 10.1007/s00251-002-0525-x

PubMed ID

  • 12557057
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Additional Document Info

start page

  • 705

end page

  • 713

volume

  • 54

issue

  • 10

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