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Monoclonal antibodies to Rho(D)-antigen of the Rhesus system. Obtaining the EBV-transformed B-lymphoblastoid human cell lines secreting IgG-anti-D antibodies [Article in Russian]

Academic Article
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Overview

authors

  • Deryugina, Elena
  • Olovnikova, N. I.
  • Lemeneva, L. N.
  • Belkina, E. V.
  • Udalov, G. A.
  • Chertkov, I. L.

publication date

  • 1991

journal

  • Gematologiya I Transfuziologiya  Journal

abstract

  • Human B-lymphoblastoid cell lines producing specific anti-D antibodies against Rh0(D) antigen have been established by EBV-transformation. The strong influence of the donor's immune status (serum titer of anti-D antibodies, intravenous booster injection prior to bleeding) is shown. The cell lines cloned by limiting dilution continuously produce anti-D antibodies in culture for 7 months or longer which is sufficient to support large-scale production of anti-D antibodies based on the cultivation of cryopreserved cells. Anti-D IgGl monoclonal antibodies obtained react with Rh-positive and Du cells but not with Rh-negative cells in serological tests. These human monoclonal antibodies could be useful in the prevention of Rh hemolytic disease of newborns.

subject areas

  • Antibodies, Monoclonal
  • B-Lymphocytes
  • Biotechnology
  • Cell Line, Transformed
  • Cell Transformation, Viral
  • Cells, Cultured
  • Herpesvirus 4, Human
  • Humans
  • Immunoglobulin G
  • In Vitro Techniques
  • Isoantibodies
  • Rh-Hr Blood-Group System
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Identity

International Standard Serial Number (ISSN)

  • 0234-5730

PubMed ID

  • 1661249
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Additional Document Info

start page

  • 11

end page

  • 16

volume

  • 36

issue

  • 8

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