Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Regulation of endogenous human gene expression by ligand-inducible TALE transcription factors

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

related to degree

  • Gaj, Thomas, Ph.D. in Chemical Biology, Scripps Research 2007 - 2013

authors

  • Mercer, A. C.
  • Gaj, Thomas
  • Sirk, S. J.
  • Lamb, B. M.
  • Barbas III, Carlos

publication date

  • October 2014

journal

  • ACS Synthetic Biology  Journal

abstract

  • The construction of increasingly sophisticated synthetic biological circuits is dependent on the development of extensible tools capable of providing specific control of gene expression in eukaryotic cells. Here, we describe a new class of synthetic transcription factors that activate gene expression in response to extracellular chemical stimuli. These inducible activators consist of customizable transcription activator-like effector (TALE) proteins combined with steroid hormone receptor ligand-binding domains. We demonstrate that these ligand-responsive TALE transcription factors allow for tunable and conditional control of gene activation and can be used to regulate the expression of endogenous genes in human cells. Since TALEs can be designed to recognize any contiguous DNA sequence, the conditional gene regulatory system described herein will enable the design of advanced synthetic gene networks.

subject areas

  • Base Sequence
  • DNA
  • Gene Expression Regulation
  • Gene Regulatory Networks
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Ligands
  • Protein Engineering
  • Receptors, Estrogen
  • Receptors, Progesterone
  • Recombinant Fusion Proteins
  • Synthetic Biology
  • Trans-Activators
scroll to property group menus

Research

keywords

  • TALE
  • gene regulation
  • inducible gene expression
  • steroid hormone receptor
  • transcription factor
scroll to property group menus

Identity

PubMed Central ID

  • PMC4097969

International Standard Serial Number (ISSN)

  • 2161-5063

Digital Object Identifier (DOI)

  • 10.1021/sb400114p

PubMed ID

  • 24251925
scroll to property group menus

Additional Document Info

start page

  • 723

end page

  • 730

volume

  • 3

issue

  • 10

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support