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A cyclic nucleotide-dependent chloride conductance in olfactory receptor neurons

Academic Article
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Overview

authors

  • Delay, R. J.
  • Dubin, Adrienne
  • Dionne, V. E.

publication date

  • September 1997

journal

  • Journal of Membrane Biology  Journal

abstract

  • Whole-cell membrane currents were recorded from olfactory receptor neurons from the neotenic salamander Necturus maculosus. Cyclic nucleotides, released intracellularly by flash photolysis of NPE-caged cAMP or NPE-caged cGMP, activated a transient chloride current. The chloride current could be elicited at constant voltage in the absence of extracellular Ca2+ as well as in the presence of 3 mM intracellular Ca2+, suggesting that the current did not require either voltage or Ca2+ transients for activation. The current could be elicited in the presence of the protein kinase inhibitors H-7 and H-89, and in the absence of intracellular ATP, indicating that activation was independent of protein kinase A activity. These results suggest that Necturus olfactory receptor neurons contain a novel chloride ion channel that may be directly gated by cyclic nucleotides.

subject areas

  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • Adenosine Triphosphate
  • Animals
  • Calcium
  • Chloride Channels
  • Cyclic AMP
  • Cyclic AMP-Dependent Protein Kinases
  • Cyclic GMP
  • Enzyme Inhibitors
  • In Vitro Techniques
  • Isoquinolines
  • Membrane Potentials
  • Necturus
  • Olfactory Receptor Neurons
  • Patch-Clamp Techniques
  • Photolysis
  • Sulfonamides
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Research

keywords

  • channel regulation
  • chloride conductance
  • cyclic AMP
  • cyclic nucleotide
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Identity

International Standard Serial Number (ISSN)

  • 0022-2631

Digital Object Identifier (DOI)

  • 10.1007/s002329900268

PubMed ID

  • 9309210
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Additional Document Info

start page

  • 53

end page

  • 60

volume

  • 159

issue

  • 1

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