Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Molecular dissection of guanine nucleotide dissociation inhibitor function in vivo - Rab-independent binding to membranes and role of Rab recycling factors

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Luan, P.
  • Balch, William E.
  • Emr, S. D.
  • Burd, C. G.

publication date

  • May 1999

journal

  • Journal of Biological Chemistry  Journal

abstract

  • Guanine nucleotide dissociation inhibitor (GDI) is an essential protein required for the recycling of Rab GTPases mediating the targeting and fusion of vesicles in the exocytic and endocytic pathways. Using site-directed mutagenesis of yeast GDI1, we demonstrate that amino acid residues required for Rab recognition in vitro are critical for function in vivo in Saccharomyces cerevisiae. Analysis of the effects of Rab-binding mutants on function in vivo reveals that only a small pool of recycling Rab protein is essential for growth, and that the rates of recycling of distinct Rabs are differentially sensitive to GDI. Furthermore, we find that membrane association of Gdi1p is Rab-independent. Mutant Gdi1 proteins unable to bind Rabs were able to associate with cellular membranes as efficiently as wild-type Gdi1p, yet caused a striking loss of the endogenous cytosolic Gdi1p-Rab pools leading to dominant inhibition of growth when expressed at levels of the normal, endogenous pool. These results demonstrate a potential role for a new recycling factor in the retrieval of Rab-GDP from membranes, and illustrate the importance of multiple effectors in regulating GDI function in Rab delivery and retrieval from membranes.

subject areas

  • Biological Transport
  • Cell Membrane
  • GTP-Binding Proteins
  • Guanine Nucleotide Dissociation Inhibitors
  • Mutagenesis
  • Mutation
  • Protein Binding
  • Saccharomyces cerevisiae
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.274.21.14806

PubMed ID

  • 10329679
scroll to property group menus

Additional Document Info

start page

  • 14806

end page

  • 14817

volume

  • 274

issue

  • 21

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support