Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Glycosyltransferase ST6GAL1 contributes to the regulation of pluripotency in human pluripotent stem cells

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

related to degree

  • Lee, Eveline, Ph.D. in Immunology, Scripps Research 2012 - 2017
  • Coleman, Ronald, Ph.D. in Biology, Scripps Research 2005 - 2014

authors

  • Wang, Y. C.
  • Stein, J. W.
  • Lynch, C. L.
  • Tran, H. T.
  • Lee, Eveline
  • Coleman, Ronald
  • Hatch, A.
  • Antontsev, V. G.
  • Chy, H. S.
  • O'Brien, C. M.
  • Murthy, S. K.
  • Laslett, A. L.
  • Peterson, S. E.
  • Loring, Jeanne

publication date

  • August 2015

journal

  • Scientific Reports  Journal

abstract

  • Many studies have suggested the significance of glycosyltransferase-mediated macromolecule glycosylation in the regulation of pluripotent states in human pluripotent stem cells (hPSCs). Here, we observed that the sialyltransferase ST6GAL1 was preferentially expressed in undifferentiated hPSCs compared to non-pluripotent cells. A lectin which preferentially recognizes α-2,6 sialylated galactosides showed strong binding reactivity with undifferentiated hPSCs and their glycoproteins, and did so to a much lesser extent with differentiated cells. In addition, downregulation of ST6GAL1 in undifferentiated hPSCs led to a decrease in POU5F1 (also known as OCT4) protein and significantly altered the expression of many genes that orchestrate cell morphogenesis during differentiation. The induction of cellular pluripotency in somatic cells was substantially impeded by the shRNA-mediated suppression of ST6GAL1, partially through interference with the expression of endogenous POU5F1 and SOX2. Targeting ST6GAL1 activity with a sialyltransferase inhibitor during cell reprogramming resulted in a dose-dependent reduction in the generation of human induced pluripotent stem cells (hiPSCs). Collectively, our data indicate that ST6GAL1 plays an important role in the regulation of pluripotency and differentiation in hPSCs, and the pluripotent state in human cells can be modulated using pharmacological tools to target sialyltransferase activity.

subject areas

  • Antigens, CD
  • Cell Differentiation
  • Enzyme Activation
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Glycosylation
  • Humans
  • Lectins
  • N-Acetylneuraminic Acid
  • Pluripotent Stem Cells
  • Sialyltransferases
scroll to property group menus

Identity

PubMed Central ID

  • PMC4548446

International Standard Serial Number (ISSN)

  • 2045-2322

Digital Object Identifier (DOI)

  • 10.1038/srep13317

PubMed ID

  • 26304831
scroll to property group menus

Additional Document Info

start page

  • 13317

volume

  • 5

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support