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Identification of primitive hematopoietic stem cells in mice by the limiting dilution method [Article in Russian]

Academic Article
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Overview

authors

  • Deryugina, Elena
  • Drize, N. I.
  • Sadovnikova, E. Y.
  • Chertkov, I. L.

publication date

  • 1989

journal

  • Gematologiya I Transfuziologiya  Journal

abstract

  • An absolute number of the primitive stem cells (PSC) providing for prolonged maintenance of hemopoiesis in culture was measured with the aid of the limiting dilution method. The number of the PSC was determined according to the magnitude of the "zero" class cultures, i. e. of the cultures in which CFUs could not be demonstrated after 5-8 weeks. The fitness of the method can be proved by the presence of the linear relations between the number of the explanted stem cells and the number of the PSC as well as by the passage of the regression curve through the origin of coordinates. The data on the content of the PSC functioning in vivo and in vitro turned out similar, amounting to about 100 cells per mouse femur.

subject areas

  • Animals
  • Bone Marrow Cells
  • Cell Count
  • Cells, Cultured
  • Colony-Forming Units Assay
  • Hematopoietic Stem Cells
  • In Vitro Techniques
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Inbred DBA
  • Spleen
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Identity

International Standard Serial Number (ISSN)

  • 0234-5730

PubMed ID

  • 2612852
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Additional Document Info

start page

  • 29

end page

  • 33

volume

  • 34

issue

  • 10

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