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Metabolic labeling of fucosylated glycans in developing zebrafish

Academic Article
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Overview

authors

  • Dehnert, K. W.
  • Beahm, B. J.
  • Huynh, T. T.
  • Baskin, J. M.
  • Laughlin, S. T.
  • Wang, W.
  • Wu, Peng
  • Amacher, S. L.
  • Bertozzi, C. R.

publication date

  • June 2011

journal

  • ACS Chemical Biology  Journal

abstract

  • Many developmental processes depend on proper fucosylation, but this post-translational modification is difficult to monitor in vivo. Here we applied a chemical reporter strategy to visualize fucosylated glycans in developing zebrafish. Using azide-derivatized analogues of fucose, we metabolically labeled cell-surface glycans and then detected the incorporated azides via copper-free click chemistry with a difluorinated cyclooctyne probe. We found that the fucose salvage pathway enzymes are expressed during zebrafish embryogenesis but that they process the azide-modified substrates inefficiently. We were able to bypass the salvage pathway by using an azide-functionalized analogue of GDP-fucose. This nucleotide sugar was readily accepted by fucosyltransferases and provided robust cell-surface labeling of fucosylated glycans, as determined by flow cytometry and confocal microscopy analysis. We used this technique to image fucosylated glycans in the enveloping layer of zebrafish embryos during the first 5 days of development. This work provides a method to study the biosynthesis of fucosylated glycans in vivo.

subject areas

  • Animals
  • Azides
  • Click Chemistry
  • Cyclooctanes
  • Flow Cytometry
  • Fucose
  • Microscopy, Confocal
  • Molecular Structure
  • Particle Size
  • Polysaccharides
  • Staining and Labeling
  • Surface Properties
  • Zebrafish
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Identity

PubMed Central ID

  • PMC3117394

International Standard Serial Number (ISSN)

  • 1554-8929

Digital Object Identifier (DOI)

  • 10.1021/cb100284d

PubMed ID

  • 21425872
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Additional Document Info

start page

  • 547

end page

  • 552

volume

  • 6

issue

  • 6

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