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Heat-shock response transcriptional program enables high-yield and high-quality recombinant protein production in Escherichia coli

Academic Article
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Overview

related to degree

  • Liu, Yu Patrick, Ph.D. in Chemistry, Scripps Research 2010 - 2015

authors

  • Zhang, X.
  • Liu, Yu Patrick
  • Genereux, J. C.
  • Nolan, C.
  • Singh, M.
  • Kelly, Jeffery

publication date

  • September 2014

journal

  • ACS Chemical Biology  Journal

abstract

  • The biosynthesis of soluble, properly folded recombinant proteins in large quantities from Escherichia coli is desirable for academic research and industrial protein production. The basal E. coli protein homeostasis (proteostasis) network capacity is often insufficient to efficiently fold overexpressed proteins. Herein we demonstrate that a transcriptionally reprogrammed E. coli proteostasis network is generally superior for producing soluble, folded, and functional recombinant proteins. Reprogramming is accomplished by overexpressing a negative feedback deficient heat-shock response transcription factor before and during overexpression of the protein-of-interest. The advantage of transcriptional reprogramming versus simply overexpressing select proteostasis network components (e.g., chaperones and co-chaperones, which has been explored previously) is that a large number of proteostasis network components are upregulated at their evolved stoichiometry, thus maintaining the system capabilities of the proteostasis network that are currently incompletely understood. Transcriptional proteostasis network reprogramming mediated by stress-responsive signaling in the absence of stress should also be useful for protein production in other cells.

subject areas

  • Arabinose
  • Chaperonin 10
  • Chaperonin 60
  • Escherichia coli
  • Escherichia coli Proteins
  • Feedback, Physiological
  • Gene Expression Regulation, Bacterial
  • HSP40 Heat-Shock Proteins
  • Heat-Shock Proteins
  • Heat-Shock Response
  • Kanamycin Resistance
  • Mutation
  • Protein Engineering
  • Proteomics
  • Recombinant Proteins
  • Sigma Factor
  • Temperature
  • Transcription, Genetic
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Identity

PubMed Central ID

  • PMC4168666

International Standard Serial Number (ISSN)

  • 1554-8929

Digital Object Identifier (DOI)

  • 10.1021/cb5004477

PubMed ID

  • 25051296
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Additional Document Info

start page

  • 1945

end page

  • 1949

volume

  • 9

issue

  • 9

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