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FBH1 affects warm temperature responses in the Arabidopsis circadian clock

Academic Article
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Overview

authors

  • Nagel, D. H.
  • Pruneda-Paz, J. L.
  • Kay, Steve A.

publication date

  • October 2014

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • In Arabidopsis, the circadian clock allows the plant to coordinate daily external signals with internal processes, conferring enhanced fitness and growth vigor. Although external cues such as temperature can entrain the clock, an important feature of the clock is the ability to maintain a relatively constant period over a range of physiological temperatures; this ability is referred to as "temperature compensation." However, how temperature actually is perceived and integrated into the clock molecular circuitry remains largely unknown. In an effort to identify additional regulators of the circadian clock, including putative components that could modulate the clock response to changes in environmental signals, we identified in a previous large-scale screen a transcription factor that interacts with and regulates the promoter activity of a core clock gene. In this report, we characterized this transcription factor, flowering basic helix-loop-helix 1 (FBH1) that binds in vivo to the promoter of the key clock gene circadian clock-associated 1 (CCA1) and regulates its expression. We found that upon temperature changes, overexpression of FBH1 alters the pace of CCA1 expression by causing a period shortening and thus preventing the clock from buffering against this change in temperature. Furthermore, as is consistent with the current mechanistic model of feedback loops observed in the clock regulatory network, we also determined that CCA1 binds in vivo to the FBH1 promoter and regulates its expression. Together these results establish a role for FBH1 as a transcriptional modulator of warm temperature signals and clock responses in Arabidopsis.

subject areas

  • Adaptation, Physiological
  • Arabidopsis
  • Arabidopsis Proteins
  • Basic Helix-Loop-Helix Transcription Factors
  • Circadian Clocks
  • Feedback, Physiological
  • Gene Expression Regulation, Plant
  • Genotype
  • Plants, Genetically Modified
  • Promoter Regions, Genetic
  • Protein Binding
  • Reverse Transcriptase Polymerase Chain Reaction
  • Temperature
  • Transcription Factors
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Identity

PubMed Central ID

  • PMC4210019

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.1416666111

PubMed ID

  • 25246594
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Additional Document Info

start page

  • 14595

end page

  • 14600

volume

  • 111

issue

  • 40

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