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Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing

Academic Article
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Overview

authors

  • Lee, M.
  • Kiefel, H.
  • LaJevic, M. D.
  • Macauley, Matthew
  • O'Hara, E.
  • Pan, J.
  • Paulson, James
  • Butcher, E. C.

publication date

  • October 2014

journal

  • Nature Immunology  Journal

abstract

  • Lymphocytes are recruited from blood by high-endothelial venules (HEVs). We performed transcriptomic analyses and identified molecular signatures that distinguish HEVs from capillary endothelium and that define tissue-specific HEV specialization. Capillaries expressed gene programs for vascular development. HEV-expressed genes showed enrichment for genes encoding molecules involved in immunological defense and lymphocyte migration. We identify capillary and HEV markers and candidate mechanisms for regulated recruitment of lymphocytes, including a lymph node HEV-selective transmembrane mucin; transcriptional control of functionally specialized carbohydrate ligands for lymphocyte L-selectin; HEV expression of molecules for transendothelial migration; and metabolic programs for lipid mediators of lymphocyte motility and chemotaxis. We also elucidate a carbohydrate-recognition pathway that targets B cells to intestinal lymphoid tissues, defining CD22 as a lectin-homing receptor for mucosal HEVs.

subject areas

  • Animals
  • Capillaries
  • Cell Movement
  • Endothelial Cells
  • Endothelium
  • Female
  • Flow Cytometry
  • Gene Expression Profiling
  • Gene Ontology
  • Lymph Nodes
  • Lymphocytes
  • Lymphoid Tissue
  • Male
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Microscopy, Confocal
  • Oligonucleotide Array Sequence Analysis
  • Venules
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Identity

PubMed Central ID

  • PMC4222088

International Standard Serial Number (ISSN)

  • 1529-2908

Digital Object Identifier (DOI)

  • 10.1038/ni.2983

PubMed ID

  • 25173345
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Additional Document Info

start page

  • 982

end page

  • 995

volume

  • 15

issue

  • 10

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