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Casein kinase II promotes target silencing by miRISC through direct phosphorylation of the DEAD-box RNA helicase CGH-1

Academic Article
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Overview

authors

  • Alessi, A. F.
  • Khivansara, V.
  • Han, T.
  • Freeberg, M. A.
  • Moresco, J. J.
  • Tu, P. G.
  • Montoye, E.
  • Yates III, John
  • Karp, X.
  • Kim, J. K.

publication date

  • December 2015

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • MicroRNAs (miRNAs) play essential, conserved roles in diverse developmental processes through association with the miRNA-induced silencing complex (miRISC). Whereas fundamental insights into the mechanistic framework of miRNA biogenesis and target gene silencing have been established, posttranslational modifications that affect miRISC function are less well understood. Here we report that the conserved serine/threonine kinase, casein kinase II (CK2), promotes miRISC function in Caenorhabditis elegans. CK2 inactivation results in developmental defects that phenocopy loss of miRISC cofactors and enhances the loss of miRNA function in diverse cellular contexts. Whereas CK2 is dispensable for miRNA biogenesis and the stability of miRISC cofactors, it is required for efficient miRISC target mRNA binding and silencing. Importantly, we identify the conserved DEAD-box RNA helicase, CGH-1/DDX6, as a key CK2 substrate within miRISC and demonstrate phosphorylation of a conserved N-terminal serine is required for CGH-1 function in the miRNA pathway.

subject areas

  • Animals
  • Animals, Genetically Modified
  • Blotting, Western
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins
  • Casein Kinase II
  • DEAD-box RNA Helicases
  • Gene Expression Profiling
  • MicroRNAs
  • Oligonucleotide Array Sequence Analysis
  • Phosphorylation
  • Protein Binding
  • RNA Interference
  • RNA Nucleotidyltransferases
  • RNA, Messenger
  • RNA-Induced Silencing Complex
  • Serine
  • Signal Transduction
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Research

keywords

  • CGH-1
  • casein kinase II
  • miRISC
  • microRNA
  • phosphorylation
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Identity

PubMed Central ID

  • PMC4702986

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.1509499112

PubMed ID

  • 26669440
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Additional Document Info

start page

  • E7213

end page

  • E7222

volume

  • 112

issue

  • 52

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