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DNA-directed in vitro synthesis and assembly of the form II D-ribulose-1,5-bisphosphate carboxylase/oxygenase from Rhodopseudomonas sphaeroides

Academic Article
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Overview

authors

  • Muller, E. D.
  • Kaplan, S.

publication date

  • 1985

journal

  • Journal of Bacteriology  Journal

abstract

  • A biochemical analysis of the in vitro assembly of the form II ribulose-1,5-bisphosphate carboxylase/oxygenase from Rhodopseudomonas sphaeroides after transcription and translation from cloned DNA is presented. The predominant enzymatically active oligomeric forms of the in vitro-synthesized and -assembled ribulose-1,5-bisphosphate carboxylase are tetramers and hexamers. Assembly of the monomeric subunits to form active enzyme appears to be dependent on the presence of a minimum number of subunits in the cell extract. Assembly of ribulose-1,5-bisphosphate carboxylase also was observed when the protein-synthesizing extracts were prepared from cells which were partially derepressed for ribulose-1,5-bisphosphate carboxylase expression.

subject areas

  • DNA, Bacterial
  • DNA, Recombinant
  • Kinetics
  • Macromolecular Substances
  • Oxygenases
  • Protein Biosynthesis
  • Rhodobacter sphaeroides
  • Rhodospirillum rubrum
  • Ribulose-Bisphosphate Carboxylase
  • Transcription, Genetic
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Identity

PubMed Central ID

  • PMC214872

International Standard Serial Number (ISSN)

  • 0021-9193

PubMed ID

  • 3918003
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Additional Document Info

start page

  • 307

end page

  • 313

volume

  • 161

issue

  • 1

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