Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Mammalian adaptation of influenza A(H7N9) virus is limited by a narrow genetic bottleneck

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Zaraket, H.
  • Baranovich, T.
  • Kaplan, B. S.
  • Carter, R.
  • Song, M. S.
  • Paulson, James
  • Rehg, J. E.
  • Bahl, J.
  • Crumpton, J. C.
  • Seiler, J.
  • Edmonson, M.
  • Wu, G.
  • Karlsson, E.
  • Fabrizio, T.
  • Zhu, H.
  • Guan, Y.
  • Husain, M.
  • Schultz-Cherry, S.
  • Krauss, S.
  • McBride, R.
  • Webster, R. G.
  • Govorkova, E. A.
  • Zhang, J.
  • Russell, C. J.
  • Webby, R. J.

publication date

  • April 2015

journal

  • Nature Communications  Journal

abstract

  • Human infection with avian influenza A(H7N9) virus is associated mainly with the exposure to infected poultry. The factors that allow interspecies transmission but limit human-to-human transmission are unknown. Here we show that A/Anhui/1/2013(H7N9) influenza virus infection of chickens (natural hosts) is asymptomatic and that it generates a high genetic diversity. In contrast, diversity is tightly restricted in infected ferrets, limiting further adaptation to a fully transmissible form. Airborne transmission in ferrets is accompanied by the mutations in PB1, NP and NA genes that reduce viral polymerase and neuraminidase activity. Therefore, while A(H7N9) virus can infect mammals, further adaptation appears to incur a fitness cost. Our results reveal that a tight genetic bottleneck during avian-to-mammalian transmission is a limiting factor in A(H7N9) influenza virus adaptation to mammals. This previously unrecognized biological mechanism limiting species jumps provides a measure of adaptive potential and may serve as a risk assessment tool for pandemic preparedness.

subject areas

  • Adaptation, Physiological
  • Animals
  • Asymptomatic Infections
  • Cercopithecus aethiops
  • Chickens
  • Dogs
  • Ferrets
  • Genetic Variation
  • HEK293 Cells
  • Humans
  • Influenza A Virus, H7N9 Subtype
  • Influenza in Birds
  • Influenza, Human
  • Madin Darby Canine Kidney Cells
  • Multiplex Polymerase Chain Reaction
  • Mutation
  • Neuraminidase
  • Orthomyxoviridae Infections
  • RNA, Viral
  • RNA-Binding Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • Vero Cells
  • Viral Core Proteins
  • Viral Proteins
scroll to property group menus

Identity

PubMed Central ID

  • PMC4403340

International Standard Serial Number (ISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/ncomms7553

PubMed ID

  • 25850788
scroll to property group menus

Additional Document Info

start page

  • 6553

volume

  • 6

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support