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Titer improvement and pilot-scale production of platensimycin from Streptomyces platensis SB12026

Academic Article
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Overview

authors

  • Shi, J.
  • Pan, J.
  • Liu, L.
  • Yang, D.
  • Lu, S.
  • Zhu, X.
  • Shen, Ben
  • Duan, Y.
  • Huang, Y.

publication date

  • July 2016

journal

  • Journal of Industrial Microbiology & Biotechnology  Journal

abstract

  • Platensimycin (PTM) and platencin (PTN), isolated from several strains of Streptomyces platensis are potent antibiotics against multi-drug resistant bacteria. PTM was also shown to have antidiabetic and antisteatotic activities in mouse models. Through a novel genome-mining method, we have recently identified six PTM and PTN dual-producing strains, and generated several mutants with improved production of PTM or PTN by inactivating the pathway-specific transcriptional repressor gene ptmR1. Among them, S. platensis SB12026 gave the highest titer of 310 mg/L for PTM. In this study, we now report titer improvement by medium and fermentation optimization and pilot-scale production and isolation of PTM from SB12026. The fermentation medium optimization was achieved by manipulating the carbon and nitrogen sources, as well as the inorganic salts. The highest titer of 1560 mg/L PTM was obtained in 15-L fermentors, using a formulated medium mainly containing soluble starch, soybean flour, morpholinepropanesulfonic acid sodium salt and CaCO3. In addition, a polyamide chromatographic step was applied to facilitate the purification and 45.14 g of PTM was successfully obtained from a 60 L scale fermentation. These results would speed up the future development of PTM as human medicine.
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Research

keywords

  • Fermentation medium optimization
  • Platensimycin
  • Polyamide chromatography
  • Streptomyces platensis SB12026
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Identity

PubMed Central ID

  • PMC5056013

International Standard Serial Number (ISSN)

  • 1367-5435

Digital Object Identifier (DOI)

  • 10.1007/s10295-016-1769-z

PubMed ID

  • 27126098
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Additional Document Info

start page

  • 1027

end page

  • 1035

volume

  • 43

issue

  • 7

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