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A strategy to capture and characterize the synaptic transcriptome

Academic Article
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Overview

authors

  • Puthanveettil, Sathyanarayanan
  • Antonov, I.
  • Kalachikov, S.
  • Rajasethupathy, P.
  • Choi, Y. B.
  • Kohn, A. B.
  • Citarella, M.
  • Yu, F. H.
  • Karl, K. A.
  • Kinet, M.
  • Morozova, I.
  • Russo, J. J.
  • Ju, J. Y.
  • Moroz, L. L.
  • Kandel, E. R.

publication date

  • April 2013

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Here we describe a strategy designed to identify RNAs that are actively transported to synapses during learning. Our approach is based on the characterization of RNA transport complexes carried by molecular motor kinesin. Using this strategy in Aplysia, we have identified 5,657 unique sequences consisting of both coding and noncoding RNAs from the CNS. Several of these RNAs have key roles in the maintenance of synaptic function and growth. One of these RNAs, myosin heavy chain, is critical in presynaptic sensory neurons for the establishment of long-term facilitation, but not for its persistence.

subject areas

  • Animals
  • Aplysia
  • Central Nervous System
  • Gene Expression Profiling
  • Genome
  • High-Throughput Nucleotide Sequencing
  • In Situ Hybridization
  • Kinesin
  • Long-Term Potentiation
  • Myosin Heavy Chains
  • Neurons
  • Oligonucleotide Array Sequence Analysis
  • Protein Transport
  • RNA
  • RNA Transport
  • Sequence Analysis, RNA
  • Synapses
  • Transcriptome
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Research

keywords

  • RNA transport
  • cystoskeleton
  • local protein synthesis
  • long-term memory storage
  • neurogenomics
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Identity

PubMed Central ID

  • PMC3645558

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.1304422110

PubMed ID

  • 23589870
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Additional Document Info

start page

  • 7464

end page

  • 7469

volume

  • 110

issue

  • 18

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