Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Diced electrophoresis gel assay for screening enzymes with specified activities

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Komatsu, T.
  • Hanaoka, K.
  • Adibekian, Alexander
  • Yoshioka, K.
  • Terai, T.
  • Ueno, T.
  • Kawaguchi, M.
  • Cravatt, Benjamin
  • Nagano, T.

publication date

  • April 2013

journal

  • Journal of the American Chemical Society  Journal

abstract

  • We have established the diced electrophoresis gel (DEG) assay as a proteome-wide screening tool to identify enzymes with activities of interest using turnover-based fluorescent substrates. The method utilizes the combination of native polyacrylamide gel electrophoresis (PAGE) with a multiwell-plate-based fluorometric assay to find protein spots with the specified activity. By developing fluorescent substrates that mimic the structure of neutrophil chemoattractants, we could identify enzymes involved in metabolic inactivation of the chemoattractants.

subject areas

  • Acrylic Resins
  • Animals
  • Chemotactic Factors
  • Chromatography, High Pressure Liquid
  • Electrophoresis, Polyacrylamide Gel
  • Enzymes
  • Escherichia coli
  • Esterases
  • Fluorescent Dyes
  • Fluorometry
  • Glucosidases
  • High-Throughput Screening Assays
  • Isoelectric Focusing
  • Liver
  • Mass Spectrometry
  • Mice
  • Molecular Mimicry
  • Neutrophils
  • Peptide Hydrolases
  • Proteins
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja401792d

PubMed ID

  • 23581642
scroll to property group menus

Additional Document Info

start page

  • 6002

end page

  • 6005

volume

  • 135

issue

  • 16

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support