Tet-mediated formation of 5-hydroxymethylcytosine in RNA

Academic Article

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Overview

authors

• Fu, L.
• Guerrero, C. R.
• Zhong, N.
• Amato, N. J.
• Liu, Y.
• Liu, S.
• Cai, Q.
• Ji, D.
• Jin, S. G.
• Niedernhofer, Laura
• Pfeifer, G. P.
• Xu, G. L.
• Wang, Y.

publication date

• August 2014

journal

• Journal of the American Chemical Society  Journal

abstract

• Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (∼1 per 5000 5-methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology.

subject areas

• Animals
• Cytosine
• DNA-Binding Proteins
• Dioxygenases
• Embryonic Stem Cells
• HEK293 Cells
• Humans
• Mice
• Proto-Oncogene Proteins
• RNA

Identity

PubMed Central ID

• PMC4140497

International Standard Serial Number (ISSN)

• 0002-7863

Digital Object Identifier (DOI)

• 10.1021/ja505305z

PubMed ID

• 25073028

Additional Document Info

start page

• 11582

end page

• 11585

volume

• 136

issue

• 33