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Tet-mediated formation of 5-hydroxymethylcytosine in RNA

Academic Article
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Overview

authors

  • Fu, L.
  • Guerrero, C. R.
  • Zhong, N.
  • Amato, N. J.
  • Liu, Y.
  • Liu, S.
  • Cai, Q.
  • Ji, D.
  • Jin, S. G.
  • Niedernhofer, Laura
  • Pfeifer, G. P.
  • Xu, G. L.
  • Wang, Y.

publication date

  • August 2014

journal

  • Journal of the American Chemical Society  Journal

abstract

  • Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (∼1 per 5000 5-methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology.

subject areas

  • Animals
  • Cytosine
  • DNA-Binding Proteins
  • Dioxygenases
  • Embryonic Stem Cells
  • HEK293 Cells
  • Humans
  • Mice
  • Proto-Oncogene Proteins
  • RNA
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Identity

PubMed Central ID

  • PMC4140497

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja505305z

PubMed ID

  • 25073028
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Additional Document Info

start page

  • 11582

end page

  • 11585

volume

  • 136

issue

  • 33

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