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The proper splicing of RNAi factors is critical for pericentric heterochromatin assembly in fission yeast

Academic Article
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Overview

authors

  • Kallgren, S. P.
  • Andrews, S.
  • Tadeo, X.
  • Hou, H.
  • Moresco, J. J.
  • Tu, P. G.
  • Yates III, John
  • Nagy, P. L.
  • Jia, S. T.

publication date

  • 2014

journal

  • PLoS Genetics  Journal

abstract

  • Heterochromatin preferentially assembles at repetitive DNA elements, playing roles in transcriptional silencing, recombination suppression, and chromosome segregation. The RNAi machinery is required for heterochromatin assembly in a diverse range of organisms. In fission yeast, RNA splicing factors are also required for pericentric heterochromatin assembly, and a prevailing model is that splicing factors provide a platform for siRNA generation independently of their splicing activity. Here, by screening the fission yeast deletion library, we discovered four novel splicing factors that are required for pericentric heterochromatin assembly. Sequencing total cellular RNAs from the strongest of these mutants, cwf14Δ, showed intron retention in mRNAs of several RNAi factors. Moreover, introducing cDNA versions of RNAi factors significantly restored pericentric heterochromatin in splicing mutants. We also found that mutations of splicing factors resulted in defective telomeric heterochromatin assembly and mis-splicing the mRNA of shelterin component Tpz1, and that replacement of tpz1+ with its cDNA partially rescued heterochromatin defects at telomeres in splicing mutants. Thus, proper splicing of RNAi and shelterin factors contributes to heterochromatin assembly at pericentric regions and telomeres.

subject areas

  • Carrier Proteins
  • Centromere
  • Chromosome Segregation
  • Gene Silencing
  • Heterochromatin
  • RNA Interference
  • RNA Splicing
  • RNA, Messenger
  • RNA, Small Interfering
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
  • Telomere
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Identity

PubMed Central ID

  • PMC4038458

International Standard Serial Number (ISSN)

  • 1553-7390

Digital Object Identifier (DOI)

  • 10.1371/journal.pgen.1004334

PubMed ID

  • 24874881
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Additional Document Info

start page

  • e1004334

volume

  • 10

issue

  • 5

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